Mitochondrial adaptations in denervated muscle: relationship to muscle performance.
Mitochondrial adaptations in denervated muscle: relationship to muscle performance.
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去神经肌肉中的线粒体适应：与肌肉性能的关系。
DOI：
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发表时间：
1991
影响因子：
--
通讯作者：
David A. Hood
中科院分区：
文献类型：
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作者：
K. L. Wicks;David A. Hood
我们使用大鼠单侧坐骨神经去神经模型，持续 2、5、8、14、21、28、35 和 42 天（n = 5-9 只大鼠/天），研究了慢性去神经的肌肉中线粒体的适应及其与肌肉性能的关系。在原位刺激去神经和对侧腓肠肌-跖肌期间评估达到峰值张力的时间（TPT）、二分之一松弛时间（1/2RT）和耐力表现。 42 天后，去神经支配导致肌肉质量下降 70%。 TPT 和 1/2RT 分别增加了 17% 和 30%，表明肌肉向慢速肌肉转变。细胞色素 C 氧化酶 (CYTOX)、琥珀酸脱氢酶和柠檬酸合成酶的活性在 8-14 天时下降，到 42 天时，这些酶的活性为对照的 34-58%。线粒体磷脂心磷脂较早降低，5天后逐渐降低至对照的37%。因此，在线粒体含量减少期间，磷脂的去除似乎先于酶活性的丧失。耐力表现随着酶活性和心磷脂的降低而降低。 5 天时细胞色素 c mRNA 水平降至对照的 52%。去神经导致编码核源性 CYTOX 亚基 VIc 和线粒体源性 CYTOX 亚基 III 的 mRNA 水平协调变化。然而，CYTOX 活性的变化并不总是与亚基 mRNA 水平的变化平行。因此，转录和翻译机制在去神经支配过程中调节线粒体基因表达。
We have studied mitochondrial adaptations in muscle subject to chronic denervation, and their relationship to muscle performance, using a model of unilateral sciatic nerve denervation in rats over periods of 2, 5, 8, 14, 21, 28, 35, and 42 days (n = 5-9 rats/day). Time to peak tension (TPT), one-half relaxation time (1/2RT), and endurance performance were evaluated during in situ stimulation of denervated and contralateral gastrocnemius-plantaris muscles. Denervation led to a 70% decline in muscle mass after 42 days. TPT and 1/2RT increased 17 and 30%, respectively, indicating a transformation toward slower muscle. The activities of the enzymes cytochrome-c oxidase (CYTOX), succinate dehydrogenase, and citrate synthase were decreased by 8-14 days, and by 42 days these were 34-58% of control. The mitochondrial phospholipid cardiolipin was reduced earlier, by 5 days, and gradually decreased to 37% of control. Thus phospholipid removal appears to precede the loss of enzyme activity during decreases in mitochondrial content. Endurance performance was reduced in parallel with decreases in enzyme activity and cardiolipin. Cytochrome c mRNA levels decreased to 52% of control by 5 days. Denervation resulted in coordinated changes in mRNA levels encoding the nuclear-derived CYTOX subunit VIc and the mitochondrially derived CYTOX subunit III. However, changes in CYTOX activity did not always parallel alterations in subunit mRNA levels. Thus transcriptional and translational mechanisms operate in regulating mitochondrial gene expression during denervation.
骨骼肌适应增加的收缩活动。
DOI：
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发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Williams,RS;Garcia-Moll,M;Mellor,J;Salmons,S;Harlan,W
通讯作者：
Harlan,W
肝癌单层培养物中线粒体内膜蛋白的周转。
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Hare,JF;Hodges,R
通讯作者：
Hodges,R