Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule.
Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule.
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EP-CAM：人类上皮抗原是一种同粒细胞粘附分子。
DOI：
10.1083/jcb.125.2.437
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发表时间：
1994-04
影响因子：
7.8
通讯作者：
Warnaar, S O
中科院分区：
文献类型：
--
作者：
Litvinov, S V;Velders, M P;Bakker, H A;Fleuren, G J;Warnaar, S O
由GA-733-2基因编码的上皮糖蛋白40 （EGP40，又称GA733-2、ESA、KSA和17-1A抗原）在大多数人单细胞的基底外侧细胞表面表达。该蛋白也在绝大多数癌症中表达，并作为肿瘤标志物引起了人们的关注。这种蛋白质的功能尚不清楚。我们在这里证明了EGP40是一种上皮特异性细胞间粘附分子。该分子以不依赖Ca（2+）的方式介导转染了完整EGP40 cDNA的小鼠细胞的嗜同性细胞-细胞粘附。我们测试了两种小鼠细胞系：成纤维细胞L细胞和去分化乳腺癌L153S细胞对EGP40表达的影响。EGP40蛋白的表达在转染细胞的培养中引起形态学变化——增加了转染物的细胞间粘附——并且在悬浮聚集实验中对细胞聚集行为有明显的影响。EGP40在混合细胞群体中指导分选，特别是使转染物从相应亲本细胞中分离出来。EGP40的表达抑制了ehs基质中L细胞的侵袭性集落生长，提供了生长集落细胞之间的紧密粘附。因此，EGP40可以被认为是细胞间粘附分子的新成员。尽管EGP40分子中不存在免疫球蛋白样重复序列，但其生物学行为在一定程度上类似于细胞粘附分子（CAMs）的免疫球蛋白超家族分子。在分子的一般组织结构上，EGP40和lin-12/Notch蛋白之间存在一定的相似性。讨论了这种粘附分子在上皮组织结构形成中的可能作用。为了反映分子的功能，将EGP40命名为Ep-CAM似乎是合适的。
The epithelial glycoprotein 40 (EGP40, also known as GA733-2, ESA, KSA, and the 17-1A antigen), encoded by the GA-733-2 gene, is expressed on the baso-lateral cell surface in most human simple epithelia. The protein is also expressed in the vast majority of carcinomas and has attracted attention as a tumor marker. The function of the protein is unknown. We demonstrate here that EGP40 is an epithelium-specific intercellular adhesion molecule. The molecule mediates, in a Ca(2+)- independent manner, a homophilic cell-cell adhesion of murine cells transfected with the complete EGP40 cDNA. Two murine cell lines were tested for the effects of EGP40 expression: fibroblastic L cells and dedifferentiated mammary carcinoma L153S cells. The expression of the EGP40 protein causes morphological changes in cultures of transfected cells--increasing intercellular adhesion of the transfectants--and has a clear effect on cell aggregating behavior in suspension aggregation assays. EGP40 directs sorting in mixed cell populations, in particular, causes segregation of the transfectants from the corresponding parental cells. EGP40 expression suppresses invasive colony growth of L cells in EHS-matrigel providing tight adhesions between cells in growing colonies. EGP40 can thus be considered a new member of the intercellular adhesion molecules. In its biological behavior EGP40 resembles to some extent the molecules of the immunoglobulin superfamily of cell adhesion molecules (CAMs), although no immunoglobulin-like repeats are present in the EGP40 molecule. Certain structural similarities in general organization of the molecule exist between EGP40 and the lin-12/Notch proteins. A possible role of this adhesion molecule in formation of architecture of epithelial tissues is discussed. To reflect the function of the molecule the name Ep-CAM for EGP40 seems appropriate.