Glucose sensor MdHXK1 activates an immune response to the fungal pathogen Botryosphaeria dothidea in apple.
Glucose sensor MdHXK1 activates an immune response to the fungal pathogen Botryosphaeria dothidea in apple.
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DOI：
10.1111/ppl.13596
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发表时间：
2021-11
影响因子：
6.4
通讯作者：
Jian-qiang Yu;Xiu-ming Li;Wen-yan Wang;Kai-Di Gu;Cui-Hui Sun;C. You;Da-Gang Hu
中科院分区：
文献类型：
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作者：
Jian-qiang Yu;Xiu-ming Li;Wen-yan Wang;Kai-Di Gu;Cui-Hui Sun;C. You;Da-Gang Hu
糖是参与植物生长发育和防御反应的重要调节分子。尽管糖与抗病性之间的关系已被广泛讨论，但其潜在的分子机制仍未被探索。由葡萄座腔菌（Botryosphaeria dothidea，B. dothidea）是苹果的一种毁灭性病害，严重影响果实的品质和产量。本研究发现苹果果实的抗病程度与葡萄糖含量密切相关。因此，编码己糖激酶的基因MdHXK 1从苹果品种'Gala'中分离，并在防御反应期间进行表征。过量表达MdHXK 1可提高水杨酸合成相关基因的表达水平，从而增强苹果愈伤组织、叶片和果实的抗病性（植物抗毒素缺乏4，PAD 4;苯丙氨酸解氨酶，PAL;和增强的疾病易感性1，EDS 1）和信号传导（PR 1; PR 5;和PR基因的非表达子1，NPR 1）以及增加超氧化物（O2-）产生速率和过氧化氢（H2 O2）含量。总的来说，这项研究为MdHXK 1介导的葡萄糖信号调节苹果轮纹病抗性的分子机制提供了新的见解。
Sugars are essential regulatory molecules involved in plant growth and development and defense response. Although the relationship between sugars and disease resistance has been widely discussed, the underlying molecular mechanisms remain unexplored. Ring rot caused by Botryosphaeria dothidea (B. dothidea), which severely affects fruit quality and yield, is a destructive disease of apples (Malus domestica Borkh.). The present study found that the degree of disease resistance in apple fruit was closely related to glucose content. Therefore, the gene encoding a hexokinase, MdHXK1, was isolated from the apple cultivar 'Gala', and characterized during the defense response. Overexpression of MdHXK1 enhanced disease resistance in apple calli, leaves, and fruits by increasing the expression levels of genes related to salicylate (SA) synthesis (PHYTOALEXIN DEFICIENT 4, PAD4; PHENYLALANINE AMMONIA-LYASE, PAL; and ENHANCED DISEASE SUSCEPTIBILITY 1, EDS1) and signaling (PR1; PR5; and NONEXPRESSER OF PR GENES 1, NPR1) as well as increasing the superoxide (O2- ) production rate and the hydrogen peroxide (H2 O2 ) content. Overall, the study provides new insights into the MdHXK1-mediated molecular mechanisms by which glucose signaling regulates apple ring rot resistance.