NEURAL CELL-ADHESION MOLECULES AND MYELIN-ASSOCIATED GLYCOPROTEIN SHARE A COMMON CARBOHYDRATE MOIETY RECOGNIZED BY MONOCLONAL ANTIBODY-L2 AND ANTIBODY-HNK-1
NEURAL CELL-ADHESION MOLECULES AND MYELIN-ASSOCIATED GLYCOPROTEIN SHARE A COMMON CARBOHYDRATE MOIETY RECOGNIZED BY MONOCLONAL ANTIBODY-L2 AND ANTIBODY-HNK-1
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DOI：
10.1038/311153a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
SCHACHNER, M
中科院分区：
文献类型：
--
作者：
KRUSE, J;MAILHAMMER, R;SCHACHNER, M
细胞表面分子与神经系统形成的细胞相互作用有关。这些分子的功能特性的分析得益于抗体和细胞培养系统的组合使用。有人认为，这些分子之间的相互作用调节细胞间的相互作用在关键的发展阶段1，2。在小鼠中，N-CAM 1，3和L1抗原4已被证明介导神经细胞间的钙非依赖性粘附。N-CAM在神经突的成束和神经肌肉接头的形成中起作用。L1显然不参与突触发生，但在发展中的小鼠小脑皮质颗粒细胞神经元的迁移。这两种抗原是不同的分子和功能实体，在神经母细胞瘤6和出生后早期小脑细胞7的聚集中协同作用。考虑到两组分子之间在功能上的某种相似性，发现单克隆抗体L2可检测到结构相似性并不令人惊讶。我们在这里表明，由L2和HNK-1单克隆抗体识别的碳水化合物部分，存在于小鼠N-CAM和LI中。L2表位出现在所有主要的神经细胞类型，但不是所有的N-CAM分子表达it. This异质性点，以前未检测到的分子多样性，可能有功能的影响，调节细胞粘附在发展过程中。
Cell surface molecules have been implicated in cell interactions which underlie formation of the nervous system. The analysis of the functional properties of such molecules has profited from the combined use of antibodies and cell culture systems. It has been suggested that the interplay between these molecules modulates cell-to-cell interaction at critical developmental stages1,2. In the mouse, N-CAM1,3and L1 antigen4have been shown to mediate Ca2+-independent adhesion among neural cells. N-CAM plays a role in fasciculation of neurites and formation of neuromuscular junction. L1 is apparently not involved in synaptogenesis, but in migration of granule cell neurones in the developing mouse cerebellar cortex5. The two antigens are distinct molecular and functional entities which act synergistically in aggregation of neuroblastoma6and early postnatal cerebellar cells7. In view of a certain similarity in function between the two groups of molecules, it was not surprising to find that structural similarities are detectable by the monoclonal antibody L2. We show here that a carbohydrate moiety recognized by L2 and HNK-1 monoclonal antibodies, is present in mouse N-CAM and LI. The L2 epitope appears on all major neural cell types but not all N-CAM molecules express it. This heterogeneity points to a previously undetected molecular diversity which may have functional implications for modulating cell adhesion during development.