Influence of pH conditions on the viability of Saccharomyces boulardii yeast
Influence of pH conditions on the viability of Saccharomyces boulardii yeast
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DOI：
10.2323/jgam.54.221
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发表时间：
2008-08-01
影响因子：
1.2
通讯作者：
Charrueau, Christine
中科院分区：
文献类型：
--
作者：
Graff, Sandrine;Chaumeil, Jean-Claude;Charrueau, Christine
布拉酵母菌是一种益生菌，具有公认的健康益处。然而，其存活受到胃肠道转运的挑战，口服给药后，在粪便中回收了1 - 3%的活酵母。该研究的目的是确定酵母对胃肠道pH条件的敏感程度。因此，我们探讨了不同浓度的S。在体外模拟胃pH（pH 1.1 0.1 N HCl）和肠pH（pH 6.8磷酸盐缓冲液）的条件下，益生菌作为从水性悬浮液获得的冷冻干燥粉末商业化，对两种形式进行评价。在pH 6.8的磷酸盐缓冲液中，两种形式的S的活力保持稳定。布拉氏酵母菌培养6 h。在盐酸pH值1.1，两种形式（200毫克L-1）的活力显着下降，从5分钟。扫描/透射电子显微镜下观察显示形态损伤和破裂的酵母壁。阈值，S。布拉氏菌的活力在pH 4时不变。在最高浓度为200 g L-1时，初始pH值1.1上升到3.2，发挥保护作用。总之，尽管水悬浮酵母对酸性条件的敏感性低于冻干酵母，但对改善活S.布拉迪是必要的。
Saccharomyces boulardii is a probiotic with proven health benefits. However its survival is challenged by gastrointestinal transit, and a ratio between 1 and 3% of living yeast is recovered in the feces after oral administration. The aim of the study was to determine to what extent the yeast was sensitive to gastrointestinal pH conditions. Therefore we explored the survival of different concentrations of S. boulardii in conditions mimicking the stomach pH (pH 1.1 0.1 N HCI) and the intestinal pH (pH 6.8 phosphate buffer) in vitro. The probiotic being commercialized as a freeze-dried powder obtained from an aqueous suspension, both forms were evaluated. In phosphate buffer pH 6.8, the viability remained stable for both forms of S. boulardii for 6 h. In HCI pH 1.1, viability of both forms (200 mg L-1) significantly decreased from 5 min. Observation under scanning/transmission electron microscopy showed morphological damages and rupture of the yeast wall. Threshold value from which S. boulardii viability was unaltered was pH 4. At the highest concentration of 200 g L-1, the initial pH value of 1.1 rose to 3.2, exerting a protective effect. In conclusion, although the yeast in aqueous suspension was less sensitive than the freeze-dried yeast to acidic conditions, a gastric protection for improvement of oral bioavailability of viable S. boulardii appears necessary.