A fluorine-doped tin oxide electrode modified with gold nanoparticles for electrochemiluminescent determination of hydrogen peroxide released by living cells
A fluorine-doped tin oxide electrode modified with gold nanoparticles for electrochemiluminescent determination of hydrogen peroxide released by living cells
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金纳米粒子修饰的氟掺杂氧化锡电极用于电化学发光测定活细胞释放的过氧化氢
DOI：
10.1007/s00604-016-2051-9
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发表时间：
2017-02-01
影响因子：
5.7
通讯作者：
Zhang, Chengxiao
中科院分区：
文献类型：
--
作者：
Li, Man;Gao, Hongfang;Zhang, Chengxiao
描述了一种基于电致化学发光（ECL）的测定活细胞释放的过氧化氢（H2O2）的方法。它正在使用一种含氟的氧化锡（FTO）电极，该电极用金纳米颗粒（AuNPs）修饰。通过在FTO电极表面电沉积AuNP制备了AuNP-FTO，并用扫描电镜和电化学技术对其进行了表征。与裸FTO相比，AuNP-FTO对基于发光胺的ECL体系表现出显著的电催化活性。校准图覆盖10 nM ~ 1 μM h2o2浓度范围，检测限为8 nM（信噪比为3）。该方法能够(a)监测活细胞（包括RAW 264.7巨噬细胞和HeLa细胞）释放的药物刺激的h2o2的产生；(b) h2o2分泌抑制剂的测定。该方法由于AuNPs的强信号放大，为生物系统中h2o2的测定提供了一种可行的工具。摘要采用金纳米粒子修饰的含氟氧化锡电极，建立了电致发光法测定过氧化氢的方法。该方法可以测定活细胞释放的药物刺激下的h2o2产量和h2o2分泌抑制剂。
An electrogenerated chemiluminescence (ECL) based method is described for the determination of hydrogen peroxide (H2O2) released by living cells. It is making use of a fluorine-doped tin oxide (FTO) electrode that was modified with gold nanoparticles (AuNPs). The AuNP-FTO was fabricated by electrodeposition of AuNP on the surface of the FTO electrode and characterized by scanning electron microscopy and electrochemical techniques. The AuNP-FTO exhibits a significant electrocatalytic activity towards luminol-based ECL system compared to a bare FTO. The calibration plot covers the 10 nM to 1 μM H2O2concentration range, with a 8 nM detection limit (at a signal-to-noise ratio of 3). The method enabled (a) the monitoring of drug-stimulated production of H2O2released by living cells including RAW 264.7 macrophage cells and HeLa cells; and (b) the determination of inhibitors of H2O2secretion. The method, due to strong signal amplification by AuNPs, provides a viable tool for determination of H2O2in biological systems.Graphical abstractAn electrogenerated chemiluminescence method was developed for determination of hydrogen peroxide using a fluorine-doped tin oxide electrode modified with gold nanoparticles. This method enabled the determination of drug-stimulated production of H2O2released by living cells and inhibitor of H2O2secretion.