Preparation of Protein Conjugates via Homobifunctional Diselenoester Cross-Linker.
Preparation of Protein Conjugates via Homobifunctional Diselenoester Cross-Linker.
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通过同双功能二硒酯交联剂制备蛋白质缀合物。
DOI：
10.1021/acs.orglett.6b02568
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Guo Jun
中科院分区：
文献类型：
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作者：
Yin Xu-Guang;Gao Xiao-Fei;Du Jing-Jing;Zhang Xiao-Kang;Chen Xiang-Zhao;Wang Jian;Xin Ling-Ming;Lei Ze;Liu Zheng;Guo Jun
己二酸二烯二酯是一种有效的交联剂，可与多种小分子半抗原（包括单糖和双糖、肽、荧光染料和尼古丁）偶联共价蛋白。与n -羟基琥珀酰亚胺（NHS）和对硝基苯（PNP）连接剂相比，二烯二酯连接剂在反应性和稳定性之间取得了更好的平衡，并在温和的条件下以高的结合效率将半抗原偶联到蛋白质上。
Adipic acid diselenoester was developed as an efficient cross-linker for covalent protein conjugation with a variety of small molecular haptens, including mono- and disaccharides, peptide, fluorescence dye, and nicotine. Compared to the counterparts ofN-hydroxysuccinimide (NHS) andp-nitrophenyl (PNP) linkers, the diselenoester linker demonstrates improved balance between reactivity and stability and coupling of haptens to proteins under mild conditions with high incorporation efficiency.