Mechanisms of SARS-CoV-2 Inactivation Using UVC Laser Radiation.
Mechanisms of SARS-CoV-2 Inactivation Using UVC Laser Radiation.
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DOI：
10.1021/acsphotonics.3c00828
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发表时间：
2024-01-17
期刊：
影响因子：
7
通讯作者：
Mahajan, Sumeet
中科院分区：
文献类型：
--
作者：
Devitt, George;Johnson, Peter B.;Hanrahan, Niall;Lane, Simon I. R.;Vidale, Magdalena C.;Sheth, Bhavwanti;Allen, Joel D.;Humbert, Maria V.;Spalluto, Cosma M.;Herve, Rodolphe C.;Staples, Karl;West, Jonathan J.;Forster, Robert;Divecha, Nullin;McCormick, Christopher J.;Crispin, Max;Hempler, Nils;Malcolm, Graeme P. A.;Mahajan, Sumeet
严重急性呼吸综合征冠状病毒2（SARS-Cov-2）对人类产生了巨大的影响。通过无化学方法对表面和气溶胶进行消毒来预防传播是非常可取的。紫外线C（UVC）光具有独特的定位，可实现病原体的灭活。我们报道了紫外线辐射灭活SARS冠状病毒2并探讨其机制。使用266 nm的UVC激光的50 mJ/cm 2的剂量实现了99.89%的灭活效率，而感染性病毒体在75 mJ/cm 2下不可检测，表明>99.99%灭活。SARS-CoV-2的感染涉及由刺突糖蛋白（S）介导的病毒进入，以及依赖于其基因组翻译的病毒繁殖。我们证明了UVC辐射损伤核糖核酸（RNA），并提供了深入的表征UVC诱导的S蛋白损伤。我们发现UVC严重影响SARS-CoV- 2刺突蛋白结合人血管紧张素转换酶2（hACE 2）的能力，这与天然蛋白质构象和芳香族氨基酸完整性的丧失有关。该报告对设计和开发针对SARS-CoV-2病毒和其他病原体的快速有效的消毒系统具有重要意义。
Severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) has had a tremendous impact on humanity. Prevention of transmission by disinfection of surfaces and aerosols through a chemical-free method is highly desirable. Ultraviolet C (UVC) light is uniquely positioned to achieve inactivation of pathogens. We report the inactivation of SARS-CoV-2 virus by UVC radiation and explore its mechanisms. A dose of 50 mJ/cm2 using a UVC laser at 266 nm achieved an inactivation efficiency of 99.89%, while infectious virions were undetectable at 75 mJ/cm2 indicating >99.99% inactivation. Infection by SARS-CoV-2 involves viral entry mediated by the spike glycoprotein (S), and viral reproduction, reliant on translation of its genome. We demonstrate that UVC radiation damages ribonucleic acid (RNA) and provide in-depth characterization of UVC-induced damage of the S protein. We find that UVC severely impacts SARS-CoV- 2 spike protein’s ability to bind human angiotensin-converting enzyme 2 (hACE2) and this correlates with loss of native protein conformation and aromatic amino acid integrity. This report has important implications for the design and development of rapid and effective disinfection systems against the SARS-CoV-2 virus and other pathogens.
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影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
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DOI：
10.3390/ma10020180
发表时间：
2017-02-14
期刊：
Materials (Basel, Switzerland)
影响因子：
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作者：
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影响因子：
3.3
作者：
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通讯作者：
HAYWOOD, EK