HYL1 is required for establishment of stamen architecture with four microsporangia in Arabidopsis.
HYL1 is required for establishment of stamen architecture with four microsporangia in Arabidopsis.
复制标题
DOI：
10.1093/jxb/ert178
复制
发表时间：
2013-08
影响因子：
6.9
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
Lian H;Li X;Liu Z;He Y
在高等植物中，雄蕊产生授粉用的花粉粒。雄蕊中四个小孢子囊的协调发育是正常生育所必需的。miR 165/166-定向途径在近轴-远轴极性建立中的作用已经在叶中得到很好的定义。然而，雄蕊的近轴-远轴极性的分子机制是难以捉摸的。本文报道了低叶型叶1（HYL 1）作为microRNA（miRNA）生物合成的一般调节因子，在拟南芥四种小孢子囊雄蕊结构的建立中起着重要作用。在雄蕊中，HYL 1和miR 165/6的表达逐渐局限于侧部区域、小孢子囊、小孢子母细胞和小孢子，而HD-ZIP III基因优先在中部区域、维管束和气孔中表达。HYL 1的缺失导致每个雄蕊中形成两个而不是四个小孢子囊。在hyl 1突变体的雄蕊中，miR 165/6积累减少，而miR 165/6靶向的HD-ZIP III基因上调，FILAMENTOUS FLOWER（FIL）下调;具体而言，REVOLUTA（REV）在近轴区过表达，FIL在远轴区表达不足，伴随着两个内部小孢子囊的畸变和部分近轴化的药隔。遗传分析表明，FIL在HYL 1的下游起作用，并且hyl 1雄蕊中的缺陷部分被rev-9或phv-5 phb-6等位基因挽救。这些结果表明，HYL 1通过抑制HD-ZIP III基因和促进FIL基因通过miR 165/6调节内部小孢子囊和雄蕊结构。因此，HYL 1在雄蕊结构建立中的作用提供了对雄性育性分子机制的深入了解。
The stamen produces pollen grains for pollination in higher plants. Coordinated development of four microsporangia in the stamen is essential for normal fertility. The roles of miR165/166-directed pathways in the establishment of adaxial–abaxial polarity have been well defined in leaves. However, the molecular mechanism underlying the adaxial–abaxial polarity of the stamen is elusive. Here it is reported that HYPONASTIC LEAVES1 (HYL1), a general regulator of microRNA (miRNA) biogenesis, plays an essential role in establishing the stamen architecture of the four microsporangia in Arabidopsis thaliana. In stamens, HYL1 and miR165/6 expression are progressively restricted to the lateral region, microsporangia, microspore mother cells, and microspores, whereas HD-ZIP III genes are preferentially expressed in the middle region, vascular bundle, and stomium. Loss of HYL1 leads to the formation of two rather than four microsporangia in each stamen. In the stamen of the hyl1 mutant, miR165/6 accumulation is reduced, whereas miR165/6-targeted HD-ZIP III genes are up-regulated and FILAMENTOUS FLOWER (FIL) is down-regulated; and, specifically, REVOLUTA (REV) is overexpressed in the adaxial region and FIL is underexpressed in the abaxial regions, concomitant with the aberrance of the two inner microsporangia and partial adaxialization of the connectives. Genetic analysis reveals that FIL works downstream of HYL1, and the defects in hyl1 stamens are partially rescued by rev-9 or phv-5 phb-6 alleles. These results suggest that HYL1 modulates inner microsporangia and stamen architecture by repression of HD-ZIP III genes and promotion of the FIL gene through miR165/6. Thus, the role of HYL1 in establishment of stamen architecture provides insight into the molecular mechanism of male fertility.
登录
查看更多内容
影响因子：
9.2
作者：
Eshed, Y;Baum, SF;Bowman, JL
通讯作者：
Bowman, JL
DOI：
10.1073/pnas.96.20.11664
发表时间：
1999-09-28
影响因子：
11.1
作者：
Schiefthaler, U;Balasubramanian, S;Schneitz, K
通讯作者：
Schneitz, K
影响因子：
9.2
作者：
Park, W;Li, JJ;Chen, XM
通讯作者：
Chen, XM
影响因子：
4.6
作者：
Dinneny, JR;Weigel, D;Yanofsky, MF
通讯作者：
Yanofsky, MF
影响因子：
23.9
作者：
Ma, Hong
通讯作者：
Ma, Hong