Expression of S100A6 and S100A4 in matched samples of human colorectal mucosa, primary colorectal adenocarcinomas and liver metastases
Expression of S100A6 and S100A4 in matched samples of human colorectal mucosa, primary colorectal adenocarcinomas and liver metastases
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DOI：
10.1159/000063812
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发表时间：
2002-01-01
期刊：
影响因子：
3.5
通讯作者：
Nakamura, H
中科院分区：
文献类型：
--
作者：
Komatsu, K;Murata, K;Nakamura, H
目的：S100蛋白家族中的S100A6和S100A4被认为与肿瘤的发生和转移有关。本研究旨在探讨S100A6和S100A4在原发结直肠腺癌(T)、癌旁正常大肠粘膜(N)和肝转移瘤(M)中的表达水平。这为我们在相同的遗传背景下直接比较S100A6和S100A4的表达水平提供了优势。方法：用免疫印迹和免疫组织化学方法检测10例结直肠腺癌患者配对的N、T和M标本中S100A6和S100A4的表达。结果：S100A6在T中的表达水平显著高于N(p<；0.05)，而S100A4的表达水平在T与N之间无显著差异，S100A6和S100A4在T与T之间的表达水平也无显著差异。结论：S100A6和S100A4的表达差异提示S100A6而不是S100A4与结直肠腺癌的发生和侵袭转移有关。
Objective: S100A6 and S100A4, two of S100 protein family, have been suggested to be associated with cancer tumorigenesis and metastasis. The aim of this study was to evaluate the expression levels of S100A6 and S100A4 in matched samples of primary human colorectal adenocarcinomas (T), adjacent normal colorectal mucosa (N) and liver metastases (M). This gave us the advantage of directly comparing levels of S100A6 and S100A4 expression within the same genetic background. Methods: In matched samples of N, T and M from 10 colorectal adenocarcinoma patients, expressions of S100A6 and S100A4 were studied by Western blot and immunohistochemical analyses using specific antibodies against each protein. Results: The expression levels of S100A6 were significantly higher in T than in N (p < 0.05), while those of S100A4 showed no difference between T and N. There were no significant differences in the expression levels of S100A6 or S100A4 between M and T. Similar results were obtained by immunohistochemical analysis. Moreover, S100A6 was stained more intensely in invading fronts than in central portions of both T and M. Conclusions: The observed differential expression of S100A6 and S100A4 suggests that S100A6, rather than S100A4, is associated with human colorectal adenocarcinoma tumorigenesis and invasion/metastasis.