Chromosome replication patternsin the hyperthermophilic euryarchaea Archaeoglobus fulgidus and Methanocaldococcus (Methanococcus) jannaschii
Chromosome replication patternsin the hyperthermophilic euryarchaea Archaeoglobus fulgidus and Methanocaldococcus (Methanococcus) jannaschii
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DOI：
10.1046/j.1365-2958.2002.03111.x
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发表时间：
2002-09-01
影响因子：
3.6
通讯作者：
Bernander, R
中科院分区：
文献类型：
--
作者：
Maisnier-Patin, S;Malandrin, L;Bernander, R
利用分子标记频率分析（MFA）技术，对两种极端嗜热广古菌（Euryarchaea）：闪烁古生球菌（Archaeoglobusfulgidus）和詹氏甲烷球菌（Methanocaldococcusjannaschii）的染色体复制模式进行了分析。对于.在fulgidus中，染色体物理图谱的中心区域在指数生长期间显示出较高的基因剂量相对丰度，在基因组图谱的直径相对侧具有两个连续梯度到较低丰度的区域。这表明A.闪电鱼染色体来自单一来源。相对于cdc6，mcm和DNA聚合酶基因的推定的复制起点区域的组织不同于所报道的火球菌属物种。没有单一的复制起点或终止区可以确定为M。jannaschii，增加了这种生物体的不寻常特性。A.通过流式细胞术分析提取基因组DNA用于MFA的样品来表征闪烁藻细胞周期。细胞周期的相对长度与泉古菌相似。
We analysed chromosome replication patterns in the two hyperthermophilic euryarchaea Archaeoglobus fulgidus and < Methanocaldococcus(Methanococcus) jannaschii by marker frequency analysis (MFA). For A. fulgidus, the central region of the chromosomal physical map displayed a higher relative abundance in gene dosage during exponential growth, with two continuous gradients to a region of lower abundance at the diametrically opposite side of the genome map. This suggests bidirectional replication of the A. fulgidus chromosome from a single origin. The organization of the putative replication origin region relative to the cdc6, mcm and DNA polymerase genes differed from that reported for Pyrococcus species. No single replication origin or termination regions could be identified for M. jannaschii ,adding to the list of unusual properties of this organism. The organization of the A. fulgidus cell cycle was characterized by flow cytometry analysis of the samples from which genomic DNA was extracted for MFA. The relative lengths of the cell cycle periods were found to be similar to those of crenarchaea.