Embryonic expression of a Drosophila src gene: alternate forms of the protein are expressed in segmental stripes and in the nervous system.
Embryonic expression of a Drosophila src gene: alternate forms of the protein are expressed in segmental stripes and in the nervous system.
复制标题
果蝇 src 基因的胚胎表达：该蛋白质的替代形式在节段条纹和神经系统中表达。
DOI：
10.1101/gad.3.3.334
复制
发表时间：
1989
影响因子：
10.5
通讯作者：
Wadsworth,SC
中科院分区：
文献类型：
--
作者：
Vincent3rd,WS;Gregory,RJ;Wadsworth,SC
果蝇src相关基因Dsrc28C的表达已在蛋白水平上用单克隆抗体进行了研究。该分析表明，Dsrc28C基因编码两种蛋白质形式：从cDNA克隆序列预测的66-kD双链和另外的55-kD形式。66-kD蛋白双偶体首先在细胞囊胚阶段观察到，在发育12小时后的胚胎中无法检测到。55-kD蛋白的表达滞后于66-kD双偶体，并在整个胚胎发生过程中持续存在。间接免疫荧光显微镜显示，Dsrc28C蛋白在细胞胚层和原肠胚形成过程中定位于细胞外周。细胞外周相关染色被分解成沿完全延伸的胚芽带的外胚层条纹。条纹消失后，可见中枢和周围神经系统内大部分细胞的细胞质染色。通过表达热休克启动子驱动的全长Dsrc28C cDNA的幼虫唾液腺抗体染色显示，66-kD蛋白代表了该蛋白的细胞外周相关形式。用66-kD蛋白特异性单克隆抗体对胚胎进行染色，表明55-kD蛋白为神经系统蛋白。因此，66- kd和55-kD蛋白是Dsrc28C基因的产物，它们在胚胎中表现出不同的时间和空间表达模式。
Expression of the Drosophila src-related gene, Dsrc28C has been investigated at the protein level using monoclonal antibodies. This analysis has revealed that the Dsrc28C gene encodes two protein forms: a 66-kD doublet predicted from the sequence of a cDNA clone and an additional 55-kD form. The 66-kD protein doublet is observed first at the cellular blastoderm stage and is not detectable in embryos after 12 hr of development. Expression of the 55-kD protein lags behind that of the 66-kD doublet and then persists throughout embryogenesis. Indirect immunofluorescence microscopy reveals that Dsrc28C protein is localized to the cell periphery during cellular blastoderm and gastrulation. The cell periphery-associated staining is then resolved into ectodermal stripes along the fully extended germ band. After the stripes fade, cytoplasmic staining of the majority of cells within the central and peripheral nervous system is observed. The 66-kD protein was shown to represent the cell periphery-associated form of the protein through antibody staining of larval salivary glands expressing a heat shock promoter-driven, full-length Dsrc28C cDNA. Staining of embryos with a monoclonal antibody specific for the 66-kD protein indicates that the 55-kD protein is the nervous system form. Thus, the 66- and 55-kD proteins are products of the Dsrc28C gene, which exhibit different temporal and spatial patterns of expression in the embryo.
登录
查看更多内容
乳酸链球菌质子转位腺苷 5′-三磷酸酶的电压敏感性
影响因子：
3.5
作者：
P. Maloney;Susan C. Schattschneider
通讯作者：
Susan C. Schattschneider
牛心线粒体三磷酸腺苷酶与单价 Pi 高亲和力结合。
影响因子：
4.8
作者：
M. Kasahara;H. Penefsky
通讯作者：
H. Penefsky
线粒体质子泵细胞色素 C 氧化酶的化学计量学和热力学。
DOI：
--
发表时间：
1979
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
K. Krab;M. Wikström
通讯作者：
M. Wikström
影响因子：
3.9
作者：
Jensen,BD;Gunter,KK;Gunter,TE
通讯作者：
Gunter,TE
线粒体跨膜 pH 值和电梯度：评估其能量与呼吸频率和 5-三磷酸腺苷合成的关系。
影响因子：
2.9
作者：
D. Wilson;N. Forman
通讯作者：
N. Forman