CMOS microelectrode array for bidirectional interaction with neuronal networks
CMOS microelectrode array for bidirectional interaction with neuronal networks
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DOI：
10.1109/jssc.2006.873677
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发表时间：
2006-07-01
影响因子：
5.4
通讯作者：
Hierlemann, Andreas
中科院分区：
文献类型：
--
作者：
Heer, Flavio;Hafizovic, Sadik;Hierlemann, Andreas
提出了一种基于CMOS金属电极的体外神经元双向通信（刺激和记录）微系统。该芯片克服了限制当今双向微电极阵列的互连挑战。该微系统已在工业CMOS技术中制造，具有几个CMOS后处理步骤，以实现128个生物相容性电极，并确保芯片在生理盐水中的稳定性。该系统包括所有必要的控制电路和片内A/D和D/A转换。已实施模块化设计，其中单独的刺激和信号调节电路单元与每个电极相关联。具有8位分辨率的刺激信号可以以60 kHz的速率发送到电极的任何子集，而芯片的所有电极以20 kHz的速率连续采样。每个电极处的电路可以被单独重置到其操作点，以便抑制由刺激脉冲诱发的伪影。从培养的神经元网络的生物测量源自解离的皮层组织的受精鸡蛋的振幅高达500 uV（PP）。
A CMOS metal-electrode-based micro system for bidirectional communication (stimulation and recording) with neuronal cells in vitro is presented. The chip overcomes the interconnect challenge that limits today's bidirectional microelectrode arrays. The microsystem has been fabricated in an industrial CMOS technology with several post-CMOS processing steps to realize 128 biocompatible electrodes and to ensure chip stability in physiological saline. The system comprises all necessary control circuitry and on-chip A/D and D/A conversion. A modular design has been implemented, where individual stimulation- and signal-conditioning circuitry units are associated with each electrode. Stimulation signals with a resolution of 8 bits can be sent to any subset of electrodes at a rate of 60 kHz, while all electrodes of the chip are continuously sampled at a rate of 20 kHz. The circuitry at each electrode can be individually reset to its operating point in order to suppress artifacts evoked by the stimulation pulses. Biological measurements from cultured neuronal networks originating from dissociated cortical tissue of fertilized chicken eggs with amplitudes of up to 500 uV(PP) are presented.