Strategies for microarray analysis of limiting amounts of RNA.
Strategies for microarray analysis of limiting amounts of RNA.
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DOI：
10.1093/bfgp/2.1.31
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发表时间：
2003-04-01
期刊：
影响因子：
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通讯作者：
Livesey, F. J.
中科院分区：
文献类型：
--
作者：
Livesey, F. J.
目前用于表达分析的微阵列技术的关键限制之一是产生用于阵列分析的标记的cDNA群所需的相对大量的输入RNA。在RNA受到限制的情况下，表达谱的选择是提高cdna标记和杂交效率，或者使用扩增策略来产生足够的RNA/cDNA供标准标记方法使用。样品扩增方法必须保持起始种群中不同RNA的相对丰度，而且必须具有高度的重复性。本综述评估了当前的信号和样本扩增技术，包括那些可以用来从单个细胞产生标记的cDNA群用于阵列分析的技术。
One of the critical limitations of current microarray technologies for use in expression analyses is the relatively large amount of input RNA required to generate labelled cDNA populations for array analysis. In situations where RNA is limiting, the options for expression profiling are to increase cDNA labelling and hybridisation efficiency, or to use an amplification strategy to generate enough RNA/cDNA for use with a standard labelling method. Sample amplification approaches must preserve the representation of the relative abundances of the different RNAs within the starting population and must also be highly reproducible. This review evaluates current signal and sample amplification technologies, including those that can be used to generate labelled cDNA populations for array analysis from as little as a single cell.