Molecular typing of Giardia duodenalis in cattle, sheep and goats in an arid area of central Iran
Molecular typing of Giardia duodenalis in cattle, sheep and goats in an arid area of central Iran
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DOI：
10.1016/j.meegid.2019.104021
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发表时间：
2019-11-01
影响因子：
3.2
通讯作者：
Akrami-Mohajeri, Fateme
中科院分区：
文献类型：
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作者：
Kiani-Salmi, Narges;Fattahi-Bafghi, Ali;Akrami-Mohajeri, Fateme
十二指肠贾第鞭毛虫是人类、牲畜和野生动物最常见的肠道寄生虫之一。它对发展中国家的公共和兽医健康都具有重要意义。对伊朗亚兹德省反刍动物十二指肠贾第鞭毛虫组合进行了分子调查，对 2017 年 6 月至 11 月从屠宰的反刍动物（包括 192 头牛、192 只绵羊和 100 只山羊）的直肠收集的 484 份动物粪便样本进行了研究。对丙糖磷酸异构酶上的组合 A、B 和 E 进行了物种特异性和组合特异性 PCR对 (tpi) 基因进行了检测，并通过测序确认了贾第鞭毛虫呈阳性的样本。总共有 25 个（5.16%）粪便样本被十二指肠蠕虫感染，其中包括 8 头牛（4.2%）、12 头绵羊（6.2%）和 5 头山羊（5%）。组合特异性 PCR 在 7 个粪便样本中检测到 G. duodenalis 组合 E（6 个在绵羊中，1 个在山羊中）。未检测到组合 A 和 B。这项研究首次深入了解伊朗屠宰牲畜中的贾第鞭毛虫感染。尽管在伊朗这个炎热地区，贾第鞭毛虫感染率较低，但对农民和屠宰场工人等直接接触牲畜的人们进行有关这种人畜共患感染的教育非常重要。
Giardia duodenalis is one of the most common intestinal parasites in humans as well as livestock and wildlife. It is of both public and veterinary health importance in developing nations. A molecular survey of Giardia duodenalis assemblages in ruminants from Yazd Province, Iran was conducted on 484 animal faecal samples collected per rectum from slaughtered ruminants including 192 cattle, 192 sheep and 100 goats from June to November 2017. Species-specific and assemblage-specific PCRs for assemblages A, B and E at the triose phosphate isomerase (tpi) gene were performed, and samples positive for Giardia were confirmed by sequencing. In total, 25 (5.16%) of examined faecal samples including eight cattle (4.2%), twelve sheep (6.2%) and five goats (5%) were infected with G. duodenalis. Assemblage-specific PCR detected G. duodenalis assemblage E in seven faecal samples (six in sheep and one in a goat). Assemblages A and B were not detected. This study provides the first insight into Giardia infection in slaughtered livestock in Iran. Although the prevalence of infection with Giardia in this hotarid area of Iran was low, educating people about direct contact with livestock such as farmers and abattoirs workers about this zoonotic infection is important.