Lectin activity of Lentinus edodes

Lectin activity of Lentinus edodes
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香菇凝集素活性

DOI:
10.1007/s101230100007
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发表时间:
2001
影响因子:
3.1
通讯作者:
V. Ignatov
V. Ignatov
中科院分区:
生物学4区
文献类型:
--
作者:
O. Tsivileva;V. Nikitina;L. V. Garibova;V. Ignatov

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4株香菇菌丝体及培养液的血凝活性[L.研究了edodes(Berk.) Pegler]以寻找凝集素。培养液的血凝活性明显高于菌丝体。建立了凝集素的碳水化合物结合能力,并阐明了在几种琼脂培养基上生长的菌丝体提取物的凝集素活性与结果的关系。 L的凝集素活性。在不同的形态发生步骤中对香菇进行了检查:菌丝体、棕色菌丝膜、原基和子实体。棕色膜步骤的血凝滴度高于菌丝体,而原基和子实体步骤的活性降低。凝集素似乎参与棕色菌丝膜菌丝聚集体的形成。
The hemagglutinating activity of submerged mycelium and culture liquid for four strains ofLentinus edodes(Berk.) Sing [L. edodes(Berk.) Pegler] was studied in the search for lectins. The hemagglutinating activity of culture liquid was substantially higher, compared with mycelium. The carbohydrate-binding capacity of the agglutinins was established, and the lectin activity of extracts from mycelia grown on several agar media was elucidated in relation to fruiting. The lectin activity ofL. edodeswas examined at different morphogenetic steps: mycelium, brown mycelial film, primordium, and fruiting body. Hemagglutination titers at the brown film step were higher than in the mycelium, whereas activity at the primordial and fruiting bodies steps decreased. Lectins seem to be involved in the formation of hyphal aggregates of brown mycelial film.