Magnitude of the protonmotive force in respiring Staphylococcus aureus and Escherichia coli
Magnitude of the protonmotive force in respiring Staphylococcus aureus and Escherichia coli
复制标题
呼吸金黄色葡萄球菌和大肠杆菌的质子动力大小
DOI：
10.1128/jb.126.3.1224-1231.1976
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发表时间：
1976
影响因子：
3.2
通讯作者：
W. Hamilton
中科院分区：
文献类型：
--
作者：
S. H. Collins;W. Hamilton
估计了金黄色葡萄球菌全细胞和大肠杆菌原生质球质膜上形成的膜电位和 pH 梯度。在缬氨霉素存在下钾离子的分布和跨膜的 pH 梯度是根据从通电呼吸状态过渡到断电静息状态期间在外部介质中观察到的 pK 和 pH 的变化来确定的。在外部 pH 值约为 6.5 时，呼吸细胞中的质子动力估计为金黄色葡萄球菌为 211 mV，大肠杆菌为 230 mV。讨论了这些质子动力作为底物积累或腺苷 5'-三磷酸合成的驱动力的充分性。
The membrane potential and pH gradient developed across the plasma membranes of whole cells of Staphylococcus aureus and spheroplasts of Escherichia coli were estimated. The distributions of potassium ions in the presence of valinomycin and the pH gradient across the membrane were determined from the changes in pK and pH observed in the external medium during transition from the energized respiring state to the de-engerized resting condition. The protonmotive force in respiring cells was estimated at 211 mV for S. aureus and 230 mV for E. coli at external pH values of approximately 6.5. The adequacy of these protonmotive forces as a driving force for substrate accumulation or adenosine 5'-triphosphate synthesis is discussed.