FURTHER-STUDIES ON THE MITOGENIC RESPONSE OF CULTURED SCHWANN-CELLS TO RAT CNS AXOLEMMA-ENRICHED FRACTIONS
FURTHER-STUDIES ON THE MITOGENIC RESPONSE OF CULTURED SCHWANN-CELLS TO RAT CNS AXOLEMMA-ENRICHED FRACTIONS
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DOI：
10.1016/0165-3806(83)90112-8
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
LEWIS, BL
中科院分区：
文献类型：
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作者：
DEVRIES, GH;MINIER, LN;LEWIS, BL
从大鼠中枢神经系统中分离出富含轴膜的组分，刺激培养的雪旺细胞分裂，但不改变其形态。轴突刺激细胞的荧光激活细胞分选仪分析证明了.apprx。细胞周期 S 期雪旺细胞数量增加 3 倍。这种增加与通过光水平放射自显影观察到的[3H]胸苷标记的细胞核数量的增加密切相关。膜结合的有丝分裂原相对热稳定，但对胰蛋白酶敏感，并且通过脂质提取和超声处理而失活。与未处理的细胞相比，肝质膜没有增加有丝分裂指数，表明轴膜诱导的有丝分裂不是对外源膜的一般反应。在轴膜水平恒定的情况下增加血清浓度不会改变有丝分裂指数，这表明轴膜不会通过去除血清中的抑制因子而引起有丝分裂。神经节苷脂、髓磷脂碱性蛋白、肝素、轴突脂质提取物和 cGMP 等潜在的有丝分裂原对培养的雪旺细胞没有影响。相对于培养的雪旺细胞的其他已知有丝分裂原，讨论了轴膜相关有丝分裂因子的特征。
Axolemma-enriched fractions isolated from rat CNS stimulated cultured Schwann cells to divide without changing their morphology. Fluorescent activated cell sorter analysis of the axolemma-stimulated cells demonstrated an .apprx. 3-fold increase in the number of Schwann cells in the S-phase of the cell cycle. This increase correlated well with increases in the number of [3H]thymidine-labeled nuclei observed by light level radioautography. The membrane-bound mitogen was relatively heat-stable, but trypsin-sensitive, and was inactivated both by lipid extraction and sonication. Liver plasma membranes did not increase the mitotic index over that of untreated cells, indicating the axolemma-induced mitosis was not a general response to exogenous membranes. Increasing serum concentrations in the presence of a constant level of axolemma did not change the mitotic index, suggesting that the axolemma did not cause mitosis by removal of an inhibitory factor in serum. Potential mitogens such as gangliosides, myelin basic protein, heparin, an axolemmal lipid extract and cGMP had no effect on the cultured Schwann cells. The characteristics of the axolemma-related mitogenic factor are discussed relative to other known mitogens for cultured Schwann cells.