Combinatorial use of antibodies to secreted mycobacterial proteins in a host immune system-independent test for tuberculosis
Combinatorial use of antibodies to secreted mycobacterial proteins in a host immune system-independent test for tuberculosis
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DOI：
10.1128/jcm.39.7.2418-2424.2001
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发表时间：
2001-07-01
影响因子：
9.4
通讯作者：
Lesley, SA
中科院分区：
文献类型：
--
作者：
Landowski, CP;Godfrey, HP;Lesley, SA
结核病的实验室诊断通常是困难的，在活动性感染期间脱落的循环结核分枝杆菌蛋白的免疫检测将不依赖于完整的宿主免疫应答，并且可以利用基于抗体的测定所提供的速度和低成本，我们以前的研究表明，活动性结核病患者循环抗原85（Ag 85）蛋白水平的升高与其结核菌素皮试状态无关（S，I. Bentley-Hibbert，X，Quan，T，纽曼，Ii. Huygen和H，P，戈弗雷，Infect，Immun，67：581-588，1999）。为了将这些观察结果扩展到牛分枝杆菌BCC接种群体和另一种分泌的分枝杆菌蛋白，使用斑点免疫结合、小鼠单克隆抗BCG Ag 85复合物抗体和与分枝杆菌反应的鸡抗肽抗体，对来自97名智利结核病患者和健康对照（其中许多人在儿童时接受过BCG）的血清中的Ag 85和PstS-1（蛋白抗原B，p38抗原）进行定量。结核病Ag 85 B和PstS-1，后者的抗体已被提高到表达在大肠杆菌中的新的专有蛋白载体上的肽衍生的免疫原，通过使用任一抗Ag 85抗体测量的活动性结核病患者的血清Ag 85水平的中值显著高于健康对照（P，
Laboratory diagnosis of tuberculosis is often difficult, Immunodetection of circulating Mycobacterium tuberculosis proteins shed during active infection would not depend on an intact host immune response and could take advantage of the speed and low costs afforded by antibody-based assays, We previously showed that patients with active tuberculosis had increased levels of circulating antigen 85 (Ag85) proteins independent of their tuberculin skin test status (S, I. Bentley-Hibbert, X, Quan, T, Newman, Ii. Huygen, and H, P, Godfrey, Infect, Immun, 67:581-588, 1999), To extend these observations to a Mycobacterium bovis BCC-vaccinated population and to another secreted mycobacterial protein, Ag85 and PstS-1 (protein antigen B, p38 antigen) were quantified in sera from 97 Chilean tuberculosis patients and healthy controls (many of whom had received BCG as children) using dot immunobinding, mouse monoclonal anti-BCG Ag85 complex antibody, and chicken antipeptide antibodies reactive with M. tuberculosis Ag85B and PstS-1, The latter antibodies had been raised to peptide-derived immunogens expressed on a novel proprietary protein carrier in Escherichia coli, Median serum Ag85 levels measured by using either anti-Ag85 antibody were significantly higher in patients with active tuberculosis than in healthy controls (P,