γ‐GLUTAMYL TRANSPEPTIDASE OF RAT LIVER AND HEPATOMA TISSUES: AN ENZYME IMMUNOASSAY AND IMMUNOSTAINING STUDIES a
γ‐GLUTAMYL TRANSPEPTIDASE OF RAT LIVER AND HEPATOMA TISSUES: AN ENZYME IMMUNOASSAY AND IMMUNOSTAINING STUDIES a
复制标题
大鼠肝脏和肝癌组织的 γ-谷氨酰转肽酶：酶免疫测定和免疫染色研究
DOI：
10.1111/j.1749-6632.1983.tb32864.x
复制
发表时间：
1983
影响因子：
5.2
通讯作者：
K. Dempo
中科院分区：
文献类型：
--
作者：
N. Taniguchi;N. Yososawa;S. Iizuka;F. Sako;Y. Tsukada;M. Satoh;K. Dempo
一种灵敏的酶免疫分析方法，大鼠γ-谷氨酰转肽酶的开发，能够定量之间的5至500纳克的酶。测定了胎儿肝、成人肝、增生结节和各种组织包括肝癌的酶含量。正常大鼠肝脏中酶含量很少，而胎肝和肝癌组织中酶含量较高。本文测定了3 '-甲基-4-二甲氨基偶氮苯诱发肝癌过程中肝脏γ-谷氨酰转肽酶的含量。在肝癌发生早期含量增加，然后下降，到肝癌发展阶段又增加。酶免疫分析法测定的酶含量变化与酶法测定的变化高度一致。这表明，如前所述，酶活性的增加伴随着免疫反应性酶蛋白的增加。增生结节的免疫组化研究显示肝细胞膜中存在γ-谷氨酰转肽酶。
A sensitive enzyme immunoassay method for rat gamma-glutamyl transpeptidase was developed that was able to quantitate between 5 to 500 ng of the enzyme. The enzyme contents of fetal liver, adult liver, hyperplastic nodules, and various tissues including hepatoma were determined. Normal rat liver contained very little enzyme, while fetal liver and hepatoma tissue contained high levels. The gamma-glutamyl transpeptidase content of the liver was assayed during the process of 3'-methyl 4-dimethyl-aminoazobenzene-induced hepatocarcinogenesis. The content was increased at an early stage, then decreased, and again increased at the stage when hepatoma developed. The change of the enzyme content determined by an enzyme immunoassay was highly consistent with that determined by enzymatic assay. This indicates that the increased activity of the enzyme as previously reported was accompanied by an increased immunoreactive enzyme protein. Immunohistochemical studies on the hyperplastic nodule revealed the presence of gamma-glutamyl transpeptidase in the hepatocyte membrane.