REPLICATION STRATEGY OF HUMAN HEPATITIS-B VIRUS
REPLICATION STRATEGY OF HUMAN HEPATITIS-B VIRUS
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DOI：
10.1128/jvi.61.3.904-911.1987
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发表时间：
1987-03-01
影响因子：
5.4
通讯作者：
SCHALLER, H
中科院分区：
文献类型：
--
作者：
WILL, H;REISER, W;SCHALLER, H
为了研究人乙型肝炎病毒（hepatitis B virus，HBV）的复制策略，对人HBV RNA前基因组的5“末端和DNA正、负链的起始位点进行了定位。发现RNA前基因组末端冗余120个核苷酸;它起始于前C区，也可能作为mRNA合成主要核心蛋白和B型肝炎病毒逆转录酶。B型肝炎病毒DNA负链起始于同向重复序列DR 1内，它含有多达8个核苷酸的末端冗余，并且它的合成不需要任何模板转换。DNA正链由可能来源于RNA前基因组5“末端的短寡核糖核苷酸引发，其合成起始于直接重复序列DR 2附近。为了使其延伸通过DNA负链中的不连续性，使用该模板的末端冗余发生分子内模板转换。因此，B型肝炎病毒的逆转录和DNA复制途径与逆转录病毒的逆转录和DNA复制途径根本不同。
To study the replication strategy of the human hepatitis B virus, the 5'' end of the RNA pregenome and the initiation sites of DNA plus and minus strands have been mapped. The RNA pregenome was found to be terminally redundant by 120 nucleotides; it is initiated within the pre-C region and may also function as mRNA for synthesis of the major core protein and the hepatitis B virus reverse transcriptase. The hepatitis B virus DNA minus strand is initiated within the direct repeat sequence DR1, it contains a terminal redundancy of up to eight nucleotides, and its synthesis does not require any template switch. The DNA plus strand is primed by a short oligoribonucleotide probably derived from the 5'' end of the RNA pregenome, and its synthesis is initiated close to the direct repeat sequence DR2. For its elongation to pass the discontinuity in the DNA minus strand an intramolecular template switch occurs using the terminal redundancy of this template. Thus, the route of reverse transcription and DNA replication of hepatitis B viruses is fundamentally different from that of retroviruses.