Down regulation of lncSCIR1 after spinal cord contusion injury in rat
Down regulation of lncSCIR1 after spinal cord contusion injury in rat
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DOI：
10.1016/j.brainres.2015.07.052
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发表时间：
2015-10-22
期刊：
影响因子：
2.9
通讯作者：
Zhu, Qing
中科院分区：
文献类型：
--
作者：
Wang, Jing;Hu, Bo;Zhu, Qing
创伤性脊髓损伤(SCI)后在转录水平上发生了广泛的变化。在这项研究中，我们在大鼠挫伤脊髓损伤模型中大规模筛选了包括编码和非编码RNA物种在内的长(>；200个核苷酸)RNA转录本的表达变化。我们证实，在损伤后1、4和7天，一个长的非编码RNA(LncSCIR1)的表达显著下调。LncSCIR1基因敲除可促进星形胶质细胞在体外的增殖和迁移。我们进一步证实了lncSCIR1的下调与损伤后四种mRNAs的表达变化之间存在很强的相关性。我们的研究结果表明，lncSCIR1的下调可能在创伤性脊髓损伤的病理生理学中起着不利的作用，从而为创伤性中枢神经系统损伤的潜在治疗靶点的研究提供了新的视角。(C)2015爱思唯尔B.V.保留所有权利。
Extensive changes occur at transcriptional level after traumatic spinal cord injury (SCI). In this study, we performed a large scale screening of expression changes of long (>200 nt) RNA transcripts including both coding and non-coding RNA species in a rat contusion SCI model. We validated significant down-regulation of one long non-coding RNA (lncSCIR1) at 1, 4, and 7 days postinjury. lncSCIR1 knockdown promoted astrocyte proliferation and migration in vitro. We further validated the strong association between lncSCIR1 knock down and the expression changes of four mRNAs after injury. Our data indicated that lncSCIR1 down-regulation might play a detrimental role in the pathophysiology of traumatic SCI and thereby provided new insights into the studies of potential therapeutic targets for traumatic central nervous system (CNS) injuries. (C) 2015 Elsevier B.V. All rights reserved.