Response of the Salmon Heart Transcriptome to Pancreas Disease: Differences Between High- and Low-Ranking Families for Resistance
Response of the Salmon Heart Transcriptome to Pancreas Disease: Differences Between High- and Low-Ranking Families for Resistance
复制标题
鲑鱼心脏转录组对胰腺疾病的反应：高等级和低等级家族之间的抵抗力差异
作者：
N. Robinson;A. Krasnov;E. Burgerhout;H. Johnsen;H. Moghadam;B. Hillestad;M. Aslam;M. Baranski;S. Boison
由鲑鱼甲病毒引起的胰腺疾病导致大西洋鲑鱼养殖业遭受严重损失。我们研究的目的是更好地了解具有高和低基因组育种值（分别为 H-gEBV 和 L-gEBV）的鲑鱼在胰腺抗病性方面的生物学差异。 H-和L-gEBV家族的鱼受到腹腔注射鲑鱼甲病毒或与受感染鱼共存的攻击。共存的死亡率高于注射，L-gEBV 的死亡率高于 H-gEBV。在攻击前以及攻击后四周和十周收集心脏用于 RNA-seq 和组织病理学。第 4 周时，注射攻击的 H- 鲑鱼的心脏损伤比 L-gEBV 鱼的心脏损伤要轻。在共栖攻击后，H- 鲑鱼的病毒载量低于 L-gEBV 鲑鱼。 H-gEBV 和 L-gEBV 之间的基因表达差异在攻击前就显现出来，在第 4 周达到顶峰，并在第 10 周减弱。在第 4 周，H-gEBV 鲑鱼表现出先天抗病毒防御基因的表达较低、B 细胞和 T 细胞免疫功能受到刺激以及应激反应较弱。疾病的延迟消退解释了第 10 周时 L-gEBV 中免疫基因的较高表达。结果表明，较早动员获得性免疫可以通过加速病毒清除和疾病消退来更好地保护 H-gEBV 鲑鱼。
Pancreas disease caused by salmonid alphaviruses leads to severe losses in Atlantic salmon aquaculture. The aim of our study was to gain a better understanding of the biological differences between salmon with high and low genomic breeding values (H-gEBV and L-gEBV respectively) for pancreas disease resistance. Fish from H- and L-gEBV families were challenged by intraperitoneal injection of salmonid alphavirus or co-habitation with infected fish. Mortality was higher with co-habitation than injection, and for L- than H-gEBV. Heart for RNA-seq and histopathology was collected before challenge and at four- and ten-weeks post-challenge. Heart damage was less severe in injection-challenged H- than L-gEBV fish at week 4. Viral load was lower in H- than L-gEBV salmon after co-habitant challenge. Gene expression differences between H- and L-gEBV manifested before challenge, peaked at week 4, and moderated by week 10. At week 4, H-gEBV salmon showed lower expression of innate antiviral defence genes, stimulation of B- and T-cell immune function, and weaker stress responses. Retarded resolution of the disease explains the higher expression of immune genes in L-gEBV at week 10. Results suggest earlier mobilization of acquired immunity better protects H-gEBV salmon by accelerating clearance of the virus and resolution of the disease.