Effect and mechanism of fucoidan derivatives from Laminaria japonica in experimental adenine-induced chronic kidney disease.
Effect and mechanism of fucoidan derivatives from Laminaria japonica in experimental adenine-induced chronic kidney disease.
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DOI：
10.1016/j.jep.2011.12.022
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发表时间：
2012-02
影响因子：
5.4
通讯作者：
Jing Wang;Feng Wang;Hou Yun;Hong Zhang;Quanbin Zhang
中科院分区：
文献类型：
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作者：
Jing Wang;Feng Wang;Hou Yun;Hong Zhang;Quanbin Zhang
民族药理相关粳稻是中国流行的海鲜和药用植物，中国人用它们作为传统药物治疗水肿病，肾脏疾病的症状。材料与方法研究岩藻糖聚糖衍生物对腺嘌呤诱导的CKD大鼠的肾保护作用及其机制。岩藻糖聚糖衍生物分别以50和150mg/kg体重给药。检测血清尿素氮（SUN）、血清肌酐（SCR）及部分酶活性。结果褐藻聚糖衍生物组小鼠血清SUN和SCR水平明显降低。岩藻糖聚糖衍生物明显减轻了肾小管和肾间质的组织病理学改变，并大大减少了肾系膜面积。CKD大鼠血清酶（CAT, GSH- px）和非酶（GSH）抗氧化剂的活性/水平发生变化，同时丙二醛（MDA）水平升高。然而，褐藻聚糖衍生物的正常脂质过氧化和抗氧化防御发生。结论岩藻糖聚糖衍生物对CKD大鼠的作用机制可能是通过替代肾小球细胞的电负性含量，抑制肾小球系膜细胞增殖来缓解肾小管、间质和系膜区。研究还证实岩藻糖聚糖衍生物对CKD大鼠的作用机制与其抗氧化活性有关，能提高抗氧化酶活性、降低LPO水平的样品能缓解CKD症状。
ETHNOPHARMACOLOGICAL RELEVANCELaminaria japonica is a popular seafood and medicinal plant in China, Chinese people use them as a traditional medicine for curing edema disease, a symptom of kidney diseases.MATERIALS AND METHODSThe renal protective activity and mechanism of fucoidan derivatives was studied against adenine induced CKD in rats. The fucoidan derivatives were administered at dose of 50 and 150mg/kg body weight. The serum urea nitrogen (SUN), serum creatinine (SCR) and some enzymic activity were detected.RESULTSThe SUN and SCR level decreased significantly with fucoidan derivatives administration. Histopathological changes of renal tubules and interstitium were markedly alleviated by fucoidan derivatives and the mesangial areas were also greatly reduced. Alterations were observed in the activities/levels of serum enzymic (CAT, GSH-PX) and non-enzymic (GSH) antioxidants, along with high level of malondialdehyde (MDA) in the CKD rats. However, normalized lipid peroxidation and antioxidant defenses occurred with fucoidan derivatives administration.CONCLUSIONThis study exhibited a new mechanism of fucoidan derivatives on CKD rats, that was the samples could alleviate renal tubules, interstitium and mesangial areas mediated by replacing the electronegative content of the glomerular cells and inhibition of mesangial cell proliferation. The study also proved the mechanism of fucoidan derivatives on the CKD rats had relationship with their antioxidant activities, the samples which could enhance the activity of antioxidant enzymes and reduce the LPO level could alleviate the symptom of CKD.