A new tinman-related gene, nkx2.7, anticipates the expression of nkx2.5 and nkx2.3 in zebrafish heart and pharyngeal endoderm
A new tinman-related gene, nkx2.7, anticipates the expression of nkx2.5 and nkx2.3 in zebrafish heart and pharyngeal endoderm
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DOI：
10.1006/dbio.1996.0341
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发表时间：
1996-12-15
影响因子：
2.7
通讯作者：
Breitbart, RE
中科院分区：
文献类型：
--
作者：
Lee, KH;Xu, QH;Breitbart, RE
果蝇同源盒基因tinman及其脊椎动物同源基因Nkx-2.5和Nkx-2.3是心脏发育的关键决定因素。本文报道了一个新的tinman相关基因nkx-2.7以及Nkx-2.5和Nkx-2.3在斑马鱼中的直向同源基因。对它们在发育中的斑马鱼胚胎中的表达的分析揭示了nkx2.7转录物首先出现在前下胚层的心脏中胚层和咽内胚层前体中，在时间和空间上预测了nkx2.5和nkx2.3在这些谱系中的后期表达。nkx2.7在这些胚胎谱系中的优势与细胞命运决定中的关键作用一致，可能部分通过诱导NKX2.5和NKX2.3。这些发现提供了第一个分子线索，内胚层和心脏中胚层前体的空间组织在斑马鱼下胚层紧随原肠胚形成。他们提出了一个协调的作用，这三个tinman相关的基因在心脏和咽弓的发展，并加强果蝇和脊椎动物物种之间的基因复制和亚专业化的范例。研究结果提供了一个框架，在其中分析锡曼相关的基因表达异常斑马鱼发育过程中的潜在变化。(C)出版社：Academic Press，Inc.
The Drosophila homeobox gene tinman and its vertebrate homologs Nkx-2.5 and Nkx-2.3 are critical determinants of cardiac development, We report here the identification of a new tinman-related gene, nkx2.7, as well as orthologs of Nkx-2.5 and Nkx-2,3 in the zebrafish. Analysis of their expression in the developing zebrafish embryo reveals that nkx2.7 transcripts are the first to appear in cardiac mesodermal and pharyngeal endodermal precursors of the anterior hypoblast, anticipating both temporally and spatially the later expression of nkx2.5 and nkx2.3 in these lineages, The preeminence of nkx2.7 in these embryonic lineages is consistent with a key role in cell fate determination, perhaps in part through the induction of nkx2.5 and nkx2,3. The findings provide the first molecular clues as to the spatial organization of endodermal and cardiac mesodermal precursors in the zebrafish hypoblast immediately following gastrulation. They suggest a coordinate role for these three tinman-related genes in the development of the heart and pharyngeal arches, and reinforce the paradigm of gene duplication and subspecialization between Drosophila and vertebrate species. The results provide a framework in which to analyze potential changes in tinman-related gene expression during abnormal zebrafish development. (C) 1996 Academic Press, Inc.