Curcumin attenuates liver inflammation in ducks fed corn contaminated with ochratoxin A possibly by regulating intestinal microbiota and inhibiting toll like receptor 4/nuclear factor kappa-B signaling pathway
Curcumin attenuates liver inflammation in ducks fed corn contaminated with ochratoxin A possibly by regulating intestinal microbiota and inhibiting toll like receptor 4/nuclear factor kappa-B signaling pathway
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DOI：
10.1016/j.livsci.2023.105345
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发表时间：
2023-09-29
影响因子：
1.8
通讯作者：
Zhai，Shuang-Shuang
中科院分区：
文献类型：
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作者：
Jiang，Xia-Yu;Peng，Xin;Zhai，Shuang-Shuang
姜黄素（CUR）以其抗炎作用而闻名，并且可以调节肠道微生物组成，减轻霉菌毒素毒性。本研究旨在探讨姜黄素是否可以减轻赭曲霉毒素 A (OTA) 引起的肝脏炎症。共有 720 只混性别 1 日龄白北京鸭被随机分为 4 组：CON（对照组，不含 OTA）、OTA（饲喂 2 mg/kg OTA 污染日粮）、CUR（饲喂添加 400 mg/kg 姜黄素的日粮）和 OTA + CUR（饲喂相同 OTA 污染日粮加 400 mg/kg 姜黄素）。每个处理有6个重复，每个重复30只鸭，处理持续21 d。结果表明，OTA处理增加了肝脏白细胞介素1β（P<0.001）和白细胞介素6（IL-6）的含量（P=0.047）。补充姜黄素可减轻 OTA 诱导的肝脏 IL-6 含量（P<0.001）。宏基因组测序分析表明，CON 和 OTA 处理的鸭盲肠微生物群之间存在明显的分离。与CON处理相比，赭曲霉毒素A增加了拟杆菌的相对丰度（P<0.05）。 OTA治疗增加了与炎症和氧化应激相关的通路表达，包括脂多糖（LPS）生物合成（P=0.002）和三羧酸循环（P=0.006）。姜黄素可以减轻OTA诱导的炎症和氧化应激途径的上调（P <0.01）。与CON相比，OTA治疗增加了血清LPS含量（P<0.001）。与OTA治疗相比，OTA+CUR治疗降低了血清LPS含量（P<0.001）。与CON治疗相比，OTA治疗上调Toll样受体4（TLR4）（P=0.05）和肿瘤坏死因子-α（P=0.028）的相对mRNA表达，上调髓系分化因子88（P=0.001）和IL-6（P=0.002）的表达。与OTA处理相比，OTA+CUR处理降低了TLR4的表达（P=0.05）。姜黄素处理的TLR4mRNA表达率最低(P<0.05)。结果表明，姜黄素可以通过调节肠道微生物群来减轻 OTA 引起的炎症。此外，姜黄素对 OTA 暴露鸭的保护作用可能抑制 TLR4 信号通路。
Curcumin (CUR) has been well known for its anti-inflammatory effects, and could regulate the intestinal microbial composition, and alleviate mycotoxin toxicity. The present study was conducted to explore whether curcumin could alleviate ochratoxin A (OTA)-induced liver inflammation. A total of 720 mixed-sex 1-d-old White Pekin ducklings were randomly assigned to 4 groups: CON (control group, without OTA), OTA (fed 2mg/kg OTA-contaminated diet), CUR (ducks fed a diet with 400mg/kg curcumin), and OTA + CUR (fed the same OTA-contaminated diet plus 400mg/kg curcumin). Each treatment consisted of 6 replicates, each containing 30 ducklings, and treatment lasted for 21 d The results demonstrated that OTA treatment increased the liver interleukin-1beta (P< 0.001) and interleukin-6 (IL-6) contents (P=0.047). Curcumin supplementation alleviated OTA-induced the liver IL-6 content (P< 0.001). Metagenomic sequencing analysis indicated that a clear separation between the cecum microbiota of ducks in CON and OTA treatment. Compared with CON treatment, ochratoxin A increased the relative abundance ofBacteroides(P< 0.05). The OTA treatment increased pathway expression associated with inflammation and exposure to oxidative stress, including lipopolysaccharide (LPS) biosynthesis (P=0.002) and tricarboxylic acid cycle (P=0.006). Curcumin could alleviate the upregulation of inflammation and oxidative stress pathways induced by OTA (P <0.01). Compared with CON, OTA treatment increased serum LPS content (P< 0.001). Compared with OTA treatment, OTA + CUR treatment decreased serum LPS content (P< 0.001). Compared with the CON treatment, OTA treatment upregulated the relative mRNA expression of toll like receptor 4 (TLR4)(P=0.05) and tumor necrosis factor-alpha (P=0.028) and upregulated the expression of myeloid differentiation factor 88 (P=0.001) andIL-6(P=0.002). Compared with the OTA treatment, OTA + CUR treatment decreased the expression ofTLR4(P=0.05). The curcumin treatment had the lowest expression rate ofTLR4mRNA (P< 0.05). The results indicated that curcumin could alleviate OTA-induced inflammation by regulating the intestinal microbiota. Furthermore, the protective role of curcumin in OTA-exposed ducks possibly inhibited TLR4 signaling pathway.