Metal particle-enhanced fluorescent immunoassays on metal mirrors
Metal particle-enhanced fluorescent immunoassays on metal mirrors
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DOI：
10.1016/j.ab.2007.01.030
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发表时间：
2007-04-15
影响因子：
2.9
通讯作者：
Gryczynski, Zygmunt
中科院分区：
文献类型：
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作者：
Matveeva, Evgenia G.;Gryczynski, Ignacy;Gryczynski, Zygmunt
我们目前的荧光免疫分析的普通金属涂层表面（金属镜）增强金属纳米粒子（银岛膜[SIF]）。用SIF涂覆金属镜（用薄二氧化硅层保护的铝、金或银），并使用荧光标记的抗体在该表面上进行免疫测定（兔免疫球蛋白G或肌红蛋白免疫测定的模型测定）。我们的结果表明，单独的SIF（在没有涂覆金属的玻璃表面上）增强免疫测定信号约3至10倍。使用金属反射镜代替玻璃作为SIF的支撑，可使信号增强高达50倍。(c)2007爱思唯尔公司All rights reserved.
We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement. (c) 2007 Elsevier Inc. All rights reserved.