Extracting thermodynamic data from equilibrium melting curves for oligonucleotide order-disorder transitions.

Extracting thermodynamic data from equilibrium melting curves for oligonucleotide order-disorder transitions.
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从寡核苷酸有序-无序转变的平衡熔解曲线中提取热力学数据。

DOI:
10.1007/978-1-59259-513-6_14
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发表时间:
1994
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Breslauer,KJ
Breslauer,KJ
中科院分区:
--
文献类型:
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作者:
Breslauer,KJ

文献摘要

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大量的研究小组已经将相当大的努力集中在化学上表征核酸分子中的解链转变上。这种兴趣反映了这样一个事实,即所得到的热力学数据允许一个定义的性质的分子力,控制核酸结构,同时也提供了一个数据库,用于预测这些结构的稳定性和温度依赖性的熔化行为。
A large number of research groups have focused considerable effort toward thermodynamically characterizing melting transitions in nucleic acid molecules. This interest reflects the fact that the resulting thermodynamic data permit one to define the nature of the molecular forces that control nucleic acid structures, while also providing a data base for predicting the stability and the temperature-dependent melting behavior of these structures.