Increased expression of F1ATP synthase subunits in yeast strains carrying point mutations which destabilize the beta subunit.
Increased expression of F1ATP synthase subunits in yeast strains carrying point mutations which destabilize the beta subunit.
复制标题
携带点突变的酵母菌株中 F1ATP 合酶亚基的表达增加，从而破坏了 β 亚基的稳定性。
DOI：
10.1016/0014-5793(93)81441-2
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发表时间：
1993
期刊：
影响因子：
3.5
通讯作者：
Wiesner,RJ
中科院分区：
文献类型：
--
作者：
Aschenbrenner,M;Mueller,DM;Zak,R;Wiesner,RJ
在体外，携带ATP2点突变的酵母菌株(S.cerevisiae)的生长速度显著降低，表明该突变在体内也是有害的。该突变在体外会破坏F1的β亚单位ATP合成酶的稳定性。免疫印迹显示，突变株的β和野生型α亚单位的水平以及相应的mRNAs水平都增加了(约1.6倍)。Northern分析表明，这既是由于新的转录物物种的出现，也是由于同源转录物的上调，强烈表明这种增加可能是由于转录的激活。其他线粒体蛋白的水平，如细胞色素氧化酶，没有受到影响。我们得出的结论是，一个特定的信号将线粒体内ATP合成酶的实际表现与编码其亚单位的核基因联系起来。
In yeast strains (S. cerevisiae) carrying a point mutation of theATP2gene, which destabilizes the β subunit of F1, ATP synthase in vitro, the growth rate was reduced significantly, demonstrating that the mutation is also deleterious in vivo. Immunoblots showed that levels of the mutated β, but also of the wild-type α subunit were increased in the mutated strains, together with levels of the corresponding mRNAs (approximately 1.6-fold). Northern analysis showed that this was due to both the appearance of new transcript species as well as upregulation of the cognate transcripts, strongly indicating that the increase was probably due to activation of transcription. Levels of other mitochondrial proteins, e.g. cytochromecoxidase, were unaffected. We conclude that a specific signal communicates the actual performance of the ATP synthase inside the mitochondria to the nuclear genes encoding its subunits.