Immunohistochemical localisation of TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin in the developing baboon kidney
Immunohistochemical localisation of TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin in the developing baboon kidney
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DOI：
10.1007/s00418-008-0398-7
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发表时间：
2008-05-01
影响因子：
2.3
通讯作者：
Black, M. Jane
中科院分区：
文献类型：
--
作者：
Gubhaju, Lina;Laslett, Andrew;Black, M. Jane
狒狒是研究人类肾脏发育的理想动物模型。本研究的目的是用免疫组织化学的方法来定位抗原TRA-1-60、TRA-1-81、GCTM-2和Podocalysin在仍在发育中的狒狒肾脏和肾脏发生已完成的肾脏中的位置。用抗TRA-1-60、TRA-1-81、GCTM-2和Podocalyin的抗体对妊娠125、140、175和185天(足月=185天)狒狒的固定肾切片进行了免疫标记。在持续肾发生(妊娠125天和140天)的肾脏中，TRA-1-60、TRA-1-81和GCTM-2特异性地定位于输尿管壶腹部上皮和集合管的顶端质膜，但未检测到Podocalysin免疫染色。在肾脏发育完全(孕期175天和185天)的肾脏中，这些标志物的定位再次非常明确地定位于集合管。总而言之，尽管需要进一步的实验来确认这些抗体所标记的特定细胞类型的身份，但这项研究为通常使用的干细胞抗体在发育中的狒狒肾脏中的分布提供了新的见解。
The baboon is an ideal animal model to study human kidney development. The aim of the current study was to use immunohistochemistry to localise the antigens TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin in the developing baboon kidney where nephrogenesis was still on-going and in kidneys where nephrogenesis was complete. Fixed kidney sections from baboons delivered at 125, 140, 175 and 185 days gestation (term = 185 days) were immuno-labelled with antibodies directed against TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin. In kidneys with on-going nephrogenesis (125 and 140 days gestation), TRA-1-60, TRA-1-81 and GCTM-2 were specifically localised to the apical plasma membrane of the epithelium of the ureteric ampullae and the collecting ducts, while podocalyxin immunostaining was not detected. In kidneys where nephrogenesis was complete (175 and 185 days gestation) localisation of these markers was again very specifically localised to the collecting ducts. In conclusion, although further experimentation is required to confirm the identity of the specific cell types marked by these antibodies, this study provides new insight into the distribution of commonly utilised stem cell antibodies in the developing baboon kidney.