Sheath-flow cuvette for high-sensitivity laser-induced fluorescence detection in capillary electrophoresis
Sheath-flow cuvette for high-sensitivity laser-induced fluorescence detection in capillary electrophoresis
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DOI：
10.1366/000370207781393208
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发表时间：
2007-07-01
影响因子：
3.5
通讯作者：
Dovichi, Norman J.
中科院分区：
文献类型：
--
作者：
Sobhani, Kimia;Michels, David A.;Dovichi, Norman J.
套式样品池是高灵敏度毛细管电泳柱后激光诱导荧光检测器的关键部件。大多数设计是基于最初制造用于流式细胞仪的商业比色皿。在这些装置中，石英流动室被保持在不锈钢夹具中，该夹具难以加工并且在密封时使比色皿经受扭矩，这经常导致流动室的损坏。在这份报告中，我们提出了一个设计的比色皿，可以很容易地构建。该设计使用压缩来保持和密封石英流动室而不施加扭矩。该系统产生的检测限（3西格玛）为115 yoctomoles（70个拷贝）的碳酸酐酶标记的。
The sheath-How cuvette is a key component in a high-sensitivity post-column laser-induced fluorescence detector for capillary electrophoresis. Most designs are based on commercial cuvettes originally manufactured for use in a flow cytometer. In these devices, a quartz flow chamber is held in a stainless-steel fixture that is difficult to machine and subjects the cuvette to a torque when sealed, which frequently leads to damage of the flow chamber. In this report we present a design for a cuvette that may easily be constructed. This design uses compression to hold and seal the quartz flow chamber without applying torque. The system produces detection limits (3 sigma) of 115 yoctomoles (70 copies) for FQ-labeled carbonic anhydrase.