Graphene oxide-labeled sandwich-type impedimetric immunoassay with sensitive enhancement based on enzymatic 4-chloro-1-naphthol oxidation
Graphene oxide-labeled sandwich-type impedimetric immunoassay with sensitive enhancement based on enzymatic 4-chloro-1-naphthol oxidation
复制标题
基于酶促 4-氯-1-萘酚氧化的氧化石墨烯标记夹心型阻抗增强免疫分析
DOI：
10.1016/j.bios.2013.02.035
复制
发表时间：
2013-09-15
影响因子：
12.6
通讯作者：
Tang, Dianping
中科院分区：
文献类型：
--
作者：
Hou, Li;Cui, Yuling;Tang, Dianping
A new sandwich-type impedimetric immunosensor based on functionalized graphene oxide nanosheets with a high ratio of horseradish peroxidase (HRP) and detection antibody was developed for the detection of carcinoembryonic antigen (CEA) by coupling with enzymatic biocatalytic precipitation of 4-chloro-1-naphthol (4-CN) on the captured antibody-modified glassy carbon electrode. Two molecular tags (with and without the graphene oxide nanosheets) were investigated for the detection of CEA and improved analytical features were acquired with the graphene-based labeling. With the labeling method, the performance and factors influencing the properties of the impedimetric immunosensors were also studied and evaluated. Under the optimal conditions, the dynamic concentration range of the impedimetric immunosensors spanned from 1.0 pg mL(-1) to 80 ng mL(-1) CEA with a detection limit (LOD) of 0.64 pg mL(-1). Intra- and inter-assay coefficients of variation were less than 7.5% and 11%, respectively. Additionally, the methodology was evaluated for CEA analysis of 10 clinical serum samples and 5 diluted serum samples, receiving in a good accordance with the results obtained by the impedimetric immunoassay and the commercialized electrochemiluminescent method. (C) 2013 Elsevier B.V. All rights reserved.
