Global analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients
Global analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients
复制标题
中国前列腺癌患者差异表达miRNA的整体分析
DOI：
10.1186/1471-2164-14-757
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发表时间：
2013-11-05
期刊：
影响因子：
4.4
通讯作者：
Zhong, Wei-de
中科院分区：
文献类型：
--
作者：
He, Hui-chan;Han, Zhao-dong;Zhong, Wei-de
研究背景本研究发现，中国人前列腺癌差异表达基因的整体生理功能与其他非中国人群不同。据估计，microRNA调节超过60%的所有蛋白质编码基因的表达。为了进一步研究中国人miRNAs转录本丰度与其靶基因mRNA之间的整体关联性，我们采用microRNA微阵列技术结合生物信息学和临床病理学方法，研究中国人miRNAs的表达谱，并评估其作为诊断和预后标志物的潜力。结果显示，4例前列腺癌患者的肿瘤组织和癌旁组织中有10个差异表达的miRNAs（倍数变化≥ 1. 5;P≤ 0. 05）。与非中国人群的miRNA谱相比，本研究显示miR-23 b、miR-220、miR-221、miR-222和miR-205可能是不同人群中共同的关键治疗靶点。mRNA微阵列和miRNA微阵列的综合分析显示了特异性抑制和/或增强miRNA在基因转录水平上的作用。目前的研究还确定了15个在中国患者中特异表达的miRNAs。临床特征统计显示，miR-374 b和miR-19 a与中国前列腺癌患者的临床病理特征显著相关。miR-374 b在前列腺癌组织中下调，并且它可以被鉴定为生化无复发生存的独立预测因子。
BackgroundOur recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are estimated to regulate the expression of greater than 60% of all protein-coding genes. To further investigate the global association between the transcript abundance of miRNAs and their target mRNAs in Chinese patients, we used microRNA microarray approach combined with bioinformatics and clinical-pathological assay to investigate the miRNA profile and evaluate the potential of miRNAs as diagnostic and prognostic markers in Chinese patients.ResultsA total of 28 miRNAs (fold change ≥1.5;P≤ 0.05) were differentially expressed between tumor tissue and adjacent benign tissue of 4 prostate cancer patients.10 top Differentially expressed miRNAs were validated by qRT-PCR using all 20 tissue pairs. Compared to the miRNA profile of non-Chinese populations, the current study showed that miR-23b, miR-220, miR-221, miR-222, and miR-205 maybe common critical therapeutic targets in different populations. The integrated analysis for mRNA microarray and miRNA microarray showed the effects of specifically inhibiting and/or enhancing the function of miRNAs on the gene transcription level. The current studies also identified 15 specific expressed miRNAs in Chinese patients. The clinical feature statistics revealed that miR-374b and miR-19a have significant correlations with clinical-pathological features in Chinese patients.ConclusionsOur findings showed Chinese prostate cancer patients have a common and specific miRNA expression profile compared with non-Chinese populations. The miR-374b is down-regulated in prostate cancer tissue, and it can be identified as an independent predictor of biochemical recurrence-free survival.