SHP2 is a downstream target of ZAP70 to regulate JAK1/STAT3 and ERK signaling pathways in mouse embryonic stem cells
SHP2 is a downstream target of ZAP70 to regulate JAK1/STAT3 and ERK signaling pathways in mouse embryonic stem cells
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DOI：
10.1016/j.febslet.2010.09.016
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发表时间：
2010-10-08
期刊：
影响因子：
3.5
通讯作者：
Park, Kyung-Soon
中科院分区：
文献类型：
--
作者：
Cha, Young;Park, Kyung-Soon
以往的研究表明，ZAP70是一种Syk家族酪氨酸激酶，在小鼠胚胎干细胞(MESCs)中表达，并通过巩固SHP1的酶活性来调节Janus激酶1(JAK1)/信号转导和转录激活因子3(STAT3)信号通路。在本研究中，我们报道了SHP2是MESCs中ZAP70的另一个下游靶点。我们发现SHP2的磷酸化和酶活性受到ZAP70表达的影响。此外，我们还提供了由ZAP70和SHP2激活的ERK通路降低白血病抑制因子(LIF)受体蛋白水平的证据。根据这些结果，我们认为SHP2是ZAP70信号的重要介导者，在未分化的mESCs中调节JAK1/STAT3和ERK通路。(C)2010年欧洲生化学会联合会。爱思唯尔出版公司版权所有。
Previous research indicated that ZAP70, a Syk family tyrosine kinase, is expressed in mouse embryonic stem cells (mESCs) and regulates the Janus kinase 1 (JAK1)/signal transducer and activator of transcription 3 (STAT3) signaling through consolidating SHP1 enzymatic activity. In this study, we report that SHP2 is another downstream target of ZAP70 in mESCs. We found that SHP2 phosphorylation and enzymatic activity are affected by Zap70 expression. In addition, we present evidence that ERK pathways activated by ZAP70 and SHP2 reduce the protein level of leukemia inhibitory factor (LIF) receptor. Based on these results, we propose that SHP2 is an essential mediator of the ZAP70 signal to regulate JAK1/STAT3 and ERK pathways in undifferentiated mESCs. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.