Molecular Characterization of Fusarium Graminearum Virus 2 Isolated from Fusarium graminearum Strain 98-8-60
Molecular Characterization of Fusarium Graminearum Virus 2 Isolated from Fusarium graminearum Strain 98-8-60
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DOI：
10.5423/ppj.2011.27.3.285
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发表时间：
2011-09
影响因子：
2.3
通讯作者：
Ji Suk Yu;Kyung-Mi Lee;Moonil Son;Kook Hyung Kim
中科院分区：
文献类型：
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作者：
Ji Suk Yu;Kyung-Mi Lee;Moonil Son;Kook Hyung Kim
稻谷镰刀菌病毒2型（FgV2）感染稻谷镰刀菌菌株988-8-60，具有至少5段双链rna (dsRNAs)，标记为dsRNA-1至dsRNA-5。本研究对FgV2进行了基因组测序，并分析了其与其他分枝病毒的系统发育关系。FgV2 dsRNAs 1-5的长度从2414到3580碱基对（bp）不等。5 ‘和3 ’非翻译区（UTRs）高度保守，每个dsRNA片段分别有78-105 bp和84-306 bp的5 ‘和3 ’非翻译区。每个dsRNA片段包含一个单独的开放阅读帧（ORF）。dsRNA-1的计算机分析显示了一个假定的开放阅读框（ORF），该框显示了与含有8个保守基序的RNA依赖RNA聚合酶（RdRp）的高度序列一致性。dsRNAs 2-5也每个包含一个未知功能产物的假定ORF编码。FgV2的dsRNA-2和dsRNA-3序列分别与水稻黄斑病毒1 （MoCV1）的dsRNA-3和-4序列具有显著的同源性。在对推测的RdRp蛋白的系统发育分析中，与其他dsRNA分枝病毒相比，FgV2被发现与黄体病毒科的曲霉分枝病毒1816和MoCV1形成了一个独特的病毒分支。
Fusarium graminearum virus 2 (FgV2) infects Fusarium graminearum strain 98-8-60 and has at least five segments of double-stranded RNAs (dsRNAs), denoted as dsRNA-1 to dsRNA-5. In this study, the genome of FgV2 was sequenced and its phylogenetic relationship with other mycoviruses was analyzed. The lengths of FgV2 dsRNAs 1-5 ranged from 2414 to 3580 base pairs (bp). The 5` and 3` untranslated regions (UTRs) are highly conserved, and each dsRNA segment had 78-105 and 84-306 bp of 5` and 3` UTRs, respectively. Each dsRNA segment contained a single open reading frame (ORF). Computer analysis of dsRNA-1 revealed a putative open reading frame (ORF) that shows high sequence identity with an RNA-dependent RNA polymerase (RdRp) containing eight conserved motifs. dsRNAs 2-5 also each contain one putative ORF coding for products of unknown function. The sequences of FgV2 dsRNA-2 and dsRNA-3 have significant sequence identity with Magnaporthe oryzae chrysovirus 1 (MoCV1) dsRNA-3 and -4, respectively. When compared to other dsRNA mycoviruses in a phylogenetic analysis of the putative RdRp protein, FgV2 was found to form a distinct virus clade with Aspergillus mycovirus 1816 and MoCV1 in the family Chrysoviridae.