FimB Regulates FimA Fimbriation in Porphyromonas gingivalis
FimB Regulates FimA Fimbriation in Porphyromonas gingivalis
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DOI：
10.1177/0022034510370089
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发表时间：
2010-09-01
影响因子：
7.6
通讯作者：
Yoshimura, F.
中科院分区：
文献类型：
--
作者：
Nagano, K.;Hasegawa, Y.;Yoshimura, F.
牙周炎相关病原体牙龈卟啉单胞菌通过菌毛在牙周缝隙中定植并形成生物膜。据了解，常用菌株ATCC381和33277能产生较长的菌毛。我们在FIMA下游的FIMB中发现了一个可能的无义突变，编码了这些菌株的FIMA菌毛的一个主要亚基。事实上，除atcc 33277和381外，牙龈假单胞菌普遍表达fimb，这是fimb的基因产物。电子显微镜显示，经FIMB修复的菌株有短而密的“牙刷”状的菲玛菌毛。FIMA过表达使菌毛伸长，而FIMB过表达使菌毛缩短。FIMB修复提高了FIMA及其辅助蛋白的产量。因此，FIMB调控FIMA菌毛的长度和表达。此外，FIMB修复显著减少了细胞表面FIMA菌毛的释放，表明FIMB起到了菌毛锚的作用。修复也增强了黏附活性。
The periodontitis-associated pathogen Porphyromonas gingivalis colonizes and forms a biofilm in gingival crevices through fimbriae. It is known that the often-used strains ATCC 33277 and 381 produce long FimA fimbriae. We found a possible nonsense mutation within fimB, immediately downstream from fimA, coding a major subunit of FimA fimbriae of the strains. Indeed, P. gingivalis strains, except for ATCC 33277 and 381, universally expressed FimB, the gene product of fimB. Electron micrographs revealed that a FimB-restored strain had short and dense, "toothbrush"-like, FimA fimbriae. FimA overexpression elongated the fimbriae, whereas FimB overexpression shortened them. FimB restoration increased production of FimA and its accessory proteins. Thus, FimB regulates the length and expression of FimA fimbriae. Additionally, FimB restoration significantly reduced the release of FimA fimbriae from the cell surface, suggesting that FimB functions as an anchor of the fimbriae. The restoration enhanced adherent activity as well.