Arabidopsis Qc-SNARE genes BET11 and BET12 are required for fertility and pollen tube elongation.
Arabidopsis Qc-SNARE genes BET11 and BET12 are required for fertility and pollen tube elongation.
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DOI：
10.1186/s40529-015-0102-x
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发表时间：
2015-12
影响因子：
3.4
通讯作者：
Jauh GY
中科院分区：
文献类型：
--
作者：
Bolaños-Villegas P;Guo CL;Jauh GY
BET 11和12是花粉管伸长所必需的。花粉管是快速生长的特殊结构，以极性方式伸长。它们在将精细胞通过花的花柱组织输送到胚囊中起着至关重要的作用，在胚囊中精细胞被释放出来与卵细胞和中央细胞融合，产生胚和胚乳。花粉管顶端的极性生长被认为是由膜运输机制分泌物质引起的。在这项研究中，我们报告了拟南芥BET 11和BET 12的功能特性，这两个基因可能编码Qc-SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）。在纯合/杂合背景下的双突变体（bet 11/bet 12）表现出突变等位基因的传递减少，种子受精减少，胚胎发育缺陷，花粉管长度减少和次生花粉管形成。BET 11和BET 12都是拟南芥育性和花粉管发育所必需的。需要更多的实验来剖析所涉及的机制。本文的在线版本（doi：10.1186/s40529-015-0102-x）包含补充材料，可供授权用户使用。
BET11 and 12 are required for pollen tube elongation. Pollen tubes are rapidly growing specialized structures that elongate in a polar manner. They play a crucial role in the delivery of sperm cells through the stylar tissues of the flower and into the embryo sac, where the sperm cells are released to fuse with the egg cell and the central cell to give rise to the embryo and the endosperm. Polar growth at the pollen tube tip is believed to result from secretion of materials by membrane trafficking mechanisms. In this study, we report the functional characterization of Arabidopsis BET11 and BET12, two genes that may code for Qc-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors). Double mutants (bet11/bet12) in a homozygous/heterozygous background showed reduced transmission of the mutant alleles, reduced fertilization of seeds, defective embryo development, reduced pollen tube lengths and formation of secondary pollen tubes. Both BET11 and BET12 are required for fertility and development of pollen tubes in Arabidopsis. More experiments are required to dissect the mechanisms involved. The online version of this article (doi:10.1186/s40529-015-0102-x) contains supplementary material, which is available to authorized users.