Low-dose oral administration of human interferon alpha can control the development of Theileria parva infection in cattle
Low-dose oral administration of human interferon alpha can control the development of Theileria parva infection in cattle
复制标题
低剂量口服人干扰素α可控制牛细小泰勒虫感染的发展
作者：
A. Young;A. Maritim;D. Kariuki;D. Stagg;J. Wafula;J. Mutugi;J. Cummins;A. Richards;C. Burns
两种天然人干扰素α制剂（nHuIFN-μ [Cantell]）和（Nhuifn-μ [ISI]）用于经口给药实验感染微小泰勒虫的牛。在第一个实验中，8头黑白花公牛接种1：10稀释的T.（Marikebuni）stock.每天用1国际单位/kg体重（i.u./ kg bwt）的nHuIFN-μ（Cantell）。给予IFN的4头小牛均未发生临床泰勒虫病，但4头对照小牛中有3头死于泰勒虫病，而第4头有轻度感染。4头处理小牛中的3头和1头存活的对照小牛产生了可检测的T抗体应答。但是，在用10倍高剂量的稳定剂攻击时，存活的对照动物和4头处理的小牛中仅1头证明是免疫的。在第二项实验中，用相同的稳定剂稀释液接种4组（每组4头小牛）。三个治疗组给予1 i.u. nHuIFN-μ（Cantell），1 i.u. nHuIFN-μ（ISI）或10 i.u. nHuIFN-μ（ISI）/kg bwt，从感染后第-2天至第8天。每日一次，第四组为对照组。2头对照组发生临床泰勒虫病，2头小牛给予10 i.u. nHuINF-μ（ISI），1头小牛给予1 i.u. nHuIFN-μ（ISI），无小牛给予1 i.u. nHuIFN-μ（Cantell）/kg体重其中，各组分别有2、1、0和0头牛死亡。除2头牛未产生高抗体应答外，所有存活牛均证明对10倍高剂量稳定剂的同源攻毒具有免疫力。第三个实验使用未稀释的T. 8头公牛的细小疟原虫（Muguga）子孢子稳定（10α）。用1 i.u. nHuIFN-α（Cantell）/kg bwt和4只为对照。所有小牛均发生急性泰勒虫病，实验终止。用T.在不同浓度（0-01-100 i.u./ ml）的nHuIFN-a（Cantell）。与未处理的对照培养物相比，IFN似乎对宿主细胞或寄生虫发育变量没有影响。
Two natural human interferon alpha preparations, (nHuIFN-μ [Cantell]) and (Nhuifn-μ [ISI]), were used for the oral treatment of cattle experimentally infected with Theileria parva parva. In the first experiment, 8 Friesian bulls were inoculated with a 1 in 10 dilution of a sporozoite stabilate of T. p. parva (Marikebuni) stock. Four of the cattle were treated daily with 1 international unit/kg body weight (i.u./kg bwt) of nHuIFN-μ (Cantell) from day –2 to day 8 p.i. None of the 4 calves given IFN developed clinical theileriosis, but 3 of the 4 control calves died of theileriosis while the fourth had a mild infection. Three of 4 treated calves and the 1 surviving control calf developed a detectable antibody response to T. p. parva schizont antigen but, on challenged with a 10-fold higher dose of stabilate, the surviving control animal and only 1 of the 4 treated calves proved to be immune. In a second experiment, 4 groups of 4 calves were inoculated with the same stabilate dilution. Three treatment groups were given either 1 i.u. nHuIFN-μ (Cantell), 1 i.u. nHuIFN-μ (ISI), or 10 i.u. nHuIFN-μ (ISI)/kg bwt from day –2 to day 8 p.i. once daily and the fourth group were controls. Clinical theileriosis occurred in 2 controls, 2 calves given 10 i.u. nHuINF-μ (ISI), 1 calf given 1 i.u. nHuIFN-μ (ISI) and no calves given 1 i.u. nHuIFN-μ (Cantell)/kg bwt. Of these, 2, 1, 0 and 0 cattle died in the respective groups. All the surviving cattle proved to be immune on homologous challenge with 10-fold higher dose of stabilate except the 2 cattle which did not develop high antibody responses. A third experiment using an undiluted challenge of T. p. parva (Muguga) sporozoite stabilate (10α) on 8 steers. Four steers were treated with 1 i.u. nHuIFN-α (Cantell)/kg bwt and 4 were controls. All calves developed acute theileriosis and the experiment was terminated. Cells of the C2 lymphoblastoid cell line, infected with T. p. parva (Muguga) schizonts, were cultured in vitro with various concentrations (0-01-100 i.u./ml) of nHuIFN-a (Cantell). The IFN appeared to have no effect on host cell or parasite developmental variables when compared to untreated control cultures.
人类干扰素有多少种？
DOI：
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发表时间：
1982
期刊：
Interferon
影响因子：
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作者：
Sehgal,PB
通讯作者：
Sehgal,PB