The identification and characterization of human Sister-of-Mammalian Grainyhead (SOM) expands the grainyhead-like family of developmental transcription factors
The identification and characterization of human Sister-of-Mammalian Grainyhead (SOM) expands the grainyhead-like family of developmental transcription factors
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DOI：
10.1042/bj20021476
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发表时间：
2003-03-15
影响因子：
4.1
通讯作者：
Jane, SM
中科院分区：
文献类型：
--
作者：
Ting, SB;Wilanowski, T;Jane, SM
果蝇基因grainyhead是一个大家族的基因编码发育转录因子，是高度保守的从苍蝇到人类的创始成员。该家族由两个主要分支组成，其中一个分支的祖先基因是grainyhead，另一个分支的祖先基因是最近克隆的果蝇CP 2。我们现在扩展这个家庭的另一个新的哺乳动物成员，姐妹的哺乳动物Grainyhead（SOM），这是与Grainyhead的遗传学对齐的鉴定。SOM与其他哺乳动物的谷头蛋白同源物（包括Mammalian Grainyhead（MGR）和Brother-of-MGR）密切相关，在功能性DNA结合、蛋白质二聚化和激活结构域上与这些因子具有高度的序列同一性。蛋白质相互作用研究表明，SOM可以与MGR和Brother-of-MGR异二聚化，但不能与家族中较远的成员异二聚化。与麦穗草一样，SOM基因也通过选择性剪接产生几种具有不同功能特性的不同亚型。这些异构体的组织分布不同，并且都显示高度限制的表达模式。这些结果表明，SOM，像它的家庭成员，可能在哺乳动物的发育中发挥重要作用。
The Drosophila gene grainyhead is the founding member of a large family of genes encoding developmental transcription factors that are highly conserved from fly to human. The family consists of two main branches, with grainyhead as the ancestral gene for one branch and the recently cloned Drosophila CP2 as the ancestral gene for: the other. We now extend this family with the identification of another novel mammalian member, Sister-of-Mammalian Grainyhead (SOM), which is phylogenetically aligned with grainyhead. SOM is closely related to the other mammalian homologues of grainyhead, including Mammalian Grainyhead (MGR) and Brother-of-MGR, sharing a high degree of sequence identity with these factors in the functional DNA-binding, protein dimerization and activation domains. Protein interaction studies demonstrate that SOM can heterodimerize with MGR and Brother-of-MGR, but not with the more distant members of the family. Like grainyhead, the SOM gene too produces several distinct isoforms with differing functional properties through alternative splicing. The tissue distributions of these isoforms differ and all display highly restricted expression patterns. These findings indicate that SOM, like its family members, may play important roles in mammalian development.