Deletion and translocation of chromosome 11q13 sequences in cervical carcinoma cell lines.
Deletion and translocation of chromosome 11q13 sequences in cervical carcinoma cell lines.
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发表时间：
1995-03
影响因子：
9.8
通讯作者：
R. Jesudasan;R. Rahman;Sandeep Chandrashekharappa;G. Evans;E. Srivatsan
中科院分区：
文献类型：
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作者：
R. Jesudasan;R. Rahman;Sandeep Chandrashekharappa;G. Evans;E. Srivatsan
对HeLa细胞衍生的非致瘤性和致瘤性杂交体的分子遗传学研究此前已将HeLa细胞肿瘤抑制基因定位于11号染色体的长臂。对HeLa细胞的分子和细胞遗传学研究表明，该细胞系染色体11 q13区带发生了重排。为了确定q13重排是否是宫颈癌的非随机事件，研究了六种不同的人乳头状瘤病毒（HPV）阳性（HeLa，SiHa，Caski，C4-I，Me 180和Ms 751）和两种不同的HPV阴性（C33 A和HT 3）细胞系。使用一些q13特异性探针的长距离限制性作图显示，在三种HPV阳性细胞系（HeLa、SiHa和Caski）和一种HPV阴性细胞系（HT 3）中，INT 2探针的75 kb内出现分子重排。使用INT 2 YAC的FISH鉴定了两种细胞系HeLa和Caski中由该YAC跨越的序列内的断点。来自该YAC的INT 2粘粒在另外两个细胞系SiHa和C33 A中显示粘粒序列的缺失。然而，这两种细胞系保留了细胞周期蛋白D1的粘粒序列，该探针位于INT 2近端100 kb处。缺失是肿瘤抑制基因的标志，我们得出结论，100 kb的间隔之间的两个cosmetic可能包含宫颈癌肿瘤抑制基因的序列。
Molecular genetic studies on HeLa cell-derived nontumorigenic and tumorigenic hybrids have previously localized the HeLa cell tumor-suppressor gene to the long arm of chromosome 11. Extensive molecular and cytogenetic studies on HeLa cells have shown chromosome band 11q13 to be rearranged in this cell line. To determine whether q13 rearrangement is a nonrandom event in cervical carcinomas, six different human papilloma virus (HPV)-positive (HeLa, SiHa, Caski, C4-I, Me180, and Ms751) and two different HPV-negative (C33A and HT3) cell lines were studied. Long-range restriction mapping using a number of q13-specific probes showed molecular rearrangements within 75 kb of INT2 probe in three HPV-positive cell lines (HeLa, SiHa, and Caski) and in an HPV-negative cell line (HT3). FISH using an INT2 YAC identified a breakpoint within the sequences spanned by this YAC in two of the cell lines, HeLa and Caski. INT2 cosmid derived from this YAC showed deletion of cosmid sequences in two other cell lines, SiHa and C33A. These two cell lines, however, retained cosmid sequences of Cyclin D1, a probe localized 100 kb proximal to INT2. Deletions being the hallmark of a tumor-suppressor gene, we conclude that the 100-kb interval between the two cosmids might contain sequences of the cervical carcinoma tumor-suppressor gene.