Activation of hepatic NF-kappa B by phenobarbital in rats
Activation of hepatic NF-kappa B by phenobarbital in rats
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DOI：
10.1006/bbrc.1996.1911
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发表时间：
1996-12-24
影响因子：
3.1
通讯作者：
Glauert, HP
中科院分区：
文献类型：
--
作者：
Li, YX;Leung, LK;Glauert, HP
核因子-κ B（NF-κ B）是多种细胞中重要的应激诱导转录因子。在这项研究中，我们检查了肝肿瘤促进剂苯巴比妥是否可以激活肝脏中的NF-κ B。大鼠在饲料中喂食0.05%苯巴比妥3、6或10天。苄氧基试卤灵脱烷基酶活性在接受苯巴比妥的大鼠中大大增加。使用电泳迁移率变动分析测定NF-κ B的DNA结合活性。3天后，接受苯巴比妥的大鼠NF-κ B活性增加了两倍。在喂食苯巴比妥的大鼠中，NF-κ B活性在第6天和第10天继续增加，在第10天增加了4倍。冷竞争和抗体超迁移实验表明DNA结合活性对NF-κ B具有特异性。这些数据表明，苯巴比妥激活大鼠肝脏NF-κ B。(C)1996年学术出版社。
Nuclear factor kappa B (NF-kappa B) is an important stress-induced transcription factor in many cell types. In this study we examined if the hepatic tumor promoting agent phenobarbital could activate NF-kappa B in the liver. Rats were fed 0.05% phenobarbital in the diet for 3, 6, or 10 days. Benzyloxyresorufin dealkylase activity was greatly increased in rats receiving phenobarbital. The DNA binding activity of NF-kappa B was determined using electrophoretic mobility shift assays. After 3 days NF-kappa B activity was increased two-fold in rats receiving phenobarbital. NF-kappa B activity continued to increase at 6 and 10 days in rats fed phenobarbital, with a four-fold increase seen at day 10. Cold competition and antibody supershift experiments showed the DNA binding activity to be specific for NF-kappa B. These data show that phenobarbital activates hepatic NF-kappa B in rats. (C) 1996 Academic Press.