GLUTATHIONE S-TRANSFERASES IN HOUSEFLY (MUSCA-DOMESTICA) - LOCATION OF GST-1 AND GST-2 FAMILIES
GLUTATHIONE S-TRANSFERASES IN HOUSEFLY (MUSCA-DOMESTICA) - LOCATION OF GST-1 AND GST-2 FAMILIES
复制标题
DOI：
10.1016/0965-1748(94)00053-k
复制
发表时间：
1995-03-01
影响因子：
3.8
通讯作者：
BERGE, JB
中科院分区：
文献类型：
--
作者：
FRANCIOSA, H;BERGE, JB
用免疫组织学方法在家蝇成虫切片上分别鉴定出双翅目谷胱甘肽s -转移酶家族（GST-1和GST-2）。GST-1主要分布于血淋巴细胞，而GST-2主要分布于胸腔的间接飞行肌和中枢神经系统。在肌肉中，GST-2在细胞中的分布似乎是均匀的，而在大脑和胸椎神经节中，GST-2主要分布在由细胞体组成的皮质区。家蝇对杀虫剂的敏感品系和抗性品系的GSTs定位比较表明，抗性品系与敏感品系的GSTs定位无差异。采用酶联免疫吸附试验给药GST-2。家蝇的GST-2含量为60 pmol /只，80-90%分布在胸部，约10%分布在头部，其余分布在腹部。此外，本文还讨论了这些gst的作用，以及它们在无脊椎动物和哺乳动物中的催化活性和运输能力。
The two families of dipteran glutathione S-transferases (GST-1 and GST-2) were located separately by immunohistology on sections of adult houseflies. GST-1 was distributed in haemolymph cells, whereas GST-2 was found in the indirect flight muscles of the thorax and in the central nervous system. In the muscles, the distribution of GST-2 seemed to be uniform in cells, whereas in the brain and the thoracic ganglia GST-2 was found mainly in the cortical areas which are made up by cell bodies. Comparison of the GSTs' location between an insecticide susceptible strain of housefly and resistant ones indicated no variation due to resistance. Enzyme-linked immunosorbent assay tests were used to dose GST-2. In houseflies, there were 60 pmol of GST-2/fly, 80-90% being found in the thorax, about 10% in the head and the remainder in the abdomen. Furthermore, the roles of these GSTs are discussed in relation to their location and our knowledge on their catalytic activities or their transport ability in invertebrates and mammals.