Extensive Hair-Shaft Elongation by Isolated Mouse Whisker Follicles in Very Long-Term Gelfoam® Histoculture.
Extensive Hair-Shaft Elongation by Isolated Mouse Whisker Follicles in Very Long-Term Gelfoam® Histoculture.
复制标题
DOI：
10.1371/journal.pone.0138005
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hoffman RM
中科院分区：
文献类型：
--
作者：
Cao W;Li L;Mii S;Amoh Y;Liu F;Hoffman RM
我们之前在Gelfoam®组织培养中研究了小鼠须毛囊，以确定位于毛囊内表达巢蛋白的多能干细胞在毛囊感觉神经生长中的作用。长期Gelfoam®须组织培养使毛囊巢表达干细胞能够促进须感觉神经的广泛延伸，其中包含轴突纤维。巢蛋白启动子驱动绿色荧光蛋白（ND-GFP）的转基因小鼠被用作晶须的来源，使表达巢蛋白的干细胞在形成滤泡感觉神经时能够成像。在本报告中，我们发现Gelfoam®组织培养的须毛囊产生生长的色素和未色素毛轴。毛干长度在第4天迅速增加，并持续增长至至少第12天，之后毛干长度保持不变。到组织培养第63天，ND-GFP毛囊干细胞数量显著增加，毛囊完整。目前的研究表明，凝胶泡沫®组织培养可以支持广泛的毛干生长，以及毛囊感觉神经的生长，从分离的毛囊维持很长一段时间。Gelfoam®毛囊组织培养可以为评估促进头发生长的新药物提供很长一段时间。
We have previously studied mouse whisker follicles in Gelfoam® histoculture to determine the role of nestin-expressing plutipotent stem cells, located within the follicle, in the growth of the follicular sensory nerve. Long-term Gelfoam® whisker histoculture enabled hair follicle nestin-expressing stem cells to promote the extensive elongation of the whisker sensory nerve, which contained axon fibers. Transgenic mice in which the nestin promoter drives green fluorescent protein (ND-GFP) were used as the source of the whiskers allowing imaging of the nestin-expressing stem cells as they formed the follicular sensory nerve. In the present report, we show that Gelfoam®-histocultured whisker follicles produced growing pigmented and unpigmented hair shafts. Hair-shaft length increased rapidly by day-4 and continued growing until at least day-12 after which the hair-shaft length was constant. By day-63 in histoculture, the number of ND-GFP hair follicle stem cells increased significantly and the follicles were intact. The present study shows that Gelfoam® histoculture can support extensive hair-shaft growth as well as hair follicle sensory-nerve growth from isolated hair follicles which were maintained over very long periods of time. Gelfoam® histoculture of hair follicles can provide a very long-term period for evaluating novel agents to promote hair growth.