Requirement of the IL-2 receptor β chain for the development of Vγ3 dendritic epidermal T cells
Requirement of the IL-2 receptor β chain for the development of Vγ3 dendritic epidermal T cells
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DOI：
10.1046/j.1523-1747.1998.00214.x
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发表时间：
1998-06-01
影响因子：
6.5
通讯作者：
Ohashi, PS
中科院分区：
文献类型：
--
作者：
Kawai, K;Suzuki, H;Ohashi, PS
VγβTCR细胞在胎儿胸腺中发育，并以树突状表皮T细胞(DETC)的形式迁移到皮肤。胎儿胸腺细胞由未成熟的热稳定抗原(HSA)(高)细胞分化为成熟的HSA(低)细胞，后者主要表达IL-2受体β链(IL-2Rβ)。本研究探讨了IL-2Rβ基因缺陷小鼠体内IL-2Rβ在V-γβ细胞发育中的作用。在IL-2Rβ缺陷小鼠中，成熟的HSA(低)Vγβ胸腺细胞适度减少。在IL-2Rβ缺陷小鼠胎儿皮肤中检测到少量V-γ-β-DETC，但在新生和成年小鼠中均未检测到。这些结果提示，IL-2Rβ可能是胎儿胸腺和胎儿皮肤中Vγβ细胞存活和/或扩增的关键信号。正常小鼠胎儿表皮表达IL-15，但不表达IL-2，外源性低浓度IL-15可诱导V-γ-β-DETC增殖。胎儿Vγβ-DETC依赖于IL-2Rβ的表达和IL-15mRNA在胎儿表皮中的存在，提示IL-15信号通过IL-2Rβ在皮肤中选择性地定位这种γ-βT细胞亚群中起着重要作用。
V gamma beta TCR cells develop in the fetal thymus and migrate to the skin as dendritic epidermal T cells (DETC). Fetal V gamma beta thymocytes differentiate from immature heat stable antigen (HSA)(high) cells to mature HSA(low) cells and the latter subset predominantly expresses IL-2 receptor beta chain (IL-2R beta). In this study, the role of IL-2R beta in the development of V gamma beta cells was determined in IL-2R beta-deficient mice. There was a moderate reduction of mature HSA(low) V gamma beta thymocytes in IL-2R beta-deficient mice. Small numbers of V gamma beta DETC were detected in the fetal skin of IL-2R beta-deficient mice, but they were absent in newborn and adult mice. These results suggest that IL-2R beta may transduce the crucial signal for survival and/or expansion of V gamma beta cells in the fetal thymus and in the fetal skin. In normal mice, IL-15 but not IL-2 mRNA was expressed in the fetal epidermis and exogenous addition of low concentration of IL-15 to fetal skin organ culture induced proliferation of V gamma beta DETC. The dependence of fetal V gamma beta DETC on the expression of IL-2R beta and the presence of IL-15 mRNA in the fetal epidermis imply an essential role of IL-15 signaling through IL-2R beta in the selective localization of this gamma delta T cell subpopulation in the skin.