The Medicago truncatula CLAVATA3-LIKE CLE12/13 signaling peptides regulate nodule number depending on the CORYNE but not the COMPACT ROOT ARCHITECTURE2 receptor
The Medicago truncatula CLAVATA3-LIKE CLE12/13 signaling peptides regulate nodule number depending on the CORYNE but not the COMPACT ROOT ARCHITECTURE2 receptor
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DOI：
10.1080/15592324.2019.1598730
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发表时间：
2019-04-02
影响因子：
2.9
通讯作者：
Frugoli, Julia
中科院分区：
文献类型：
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作者：
Nowak, Stephen;Schnabel, Elise;Frugoli, Julia
我们之前表明，截形苜蓿的rdn 1和sunn超级结瘤突变体对根瘤菌CLAVAT 3/胚周围区（CLE）信号肽MtCLE 12 p和MtCLE 13 p的过表达有不同的反应，从而可以确定基因的作用顺序结瘤自动调节（AON）信号转导途径。我们测试了导致蛋白水解加工肽产生的相同基因结构（在名称后用p表示）在植物中控制根瘤数量的另外两种蛋白质的突变体（CRN和CRA 2），并且能够确定CRN参与与MtCLE 12 p和MtCLE 13 p相同的信号传导途径，而CRA 2突变体中的调节通常响应于肽，表明CRA 2可能是与SUNN、RDN 1和CRN分开的信号。基于对cra 2 -2和sunn-4双突变体的分析，我们也证实了最近的研究结果，即CRA 2在根瘤数量调控中独立于SUNN发挥作用。
We previously showed that the rdn1 and sunn supernodulation mutants of Medicago truncatula respond differentially to overexpression of the rhizobial CLAVAT3/EMBRYO SURROUNDING REGION (CLE) signaling peptides MtCLE12p and MtCLE13p, allowing the order of action of the genes to be determined in the autoregulation of nodulation (AON) signal transduction pathway. We tested the same gene constructs that lead to the production of proteolytically processed peptides (indicated by a p after the name) in plants mutant for two other proteins that control nodule number (CRN and CRA2) and were able to determine that CRN is involved in the same signaling pathway as MtCLE12p and MtCLE13p, while regulation in CRA2 mutants responds normally to the peptides, suggesting CRA2 likely signals separately from SUNN, RDN1, and CRN. Based on the analysis of the double mutant of cra2-2 and sunn-4, we also confirm recent findings that CRA2 acts independently of SUNN in nodule number regulation.