Hand-Held Raman Spectrometer-Based Dual Detection of Creatinine and Cortisol in Human Sweat Using Silver Nanoflakes
Hand-Held Raman Spectrometer-Based Dual Detection of Creatinine and Cortisol in Human Sweat Using Silver Nanoflakes
复制标题
基于手持式拉曼光谱仪的纳米银片人体汗液中肌酐和皮质醇的双重检测
DOI：
10.1021/acs.analchem.1c02496
复制
发表时间：
2021-11-05
影响因子：
7.4
通讯作者：
Hong, Yoochan
中科院分区：
文献类型：
--
作者：
Kim, Hyun Soo;Kim, Hyun Jung;Hong, Yoochan
传统的组织活检方法产生一个狭窄区域的孤立快照。因此，它不能促进疾病的全面表征和监测。近年来，从生物化学研究和临床应用的角度来看，体液中肿瘤源性成分的检测越来越受到重视。在这种情况下，由于其高灵敏度和特异性，表面增强拉曼散射（SERS）已被确定为最强大的液体活检分析技术之一。此外，它为同时检测目标提供了高容量的光谱复用，并具有获得生物分子指纹光谱的独特能力。本文介绍了用多元醇法制备银纳米雪花，并将其滴入疏水滤纸上。SERS底物由snsf和疏水滤纸组成，可以使用手持式拉曼光谱仪同时检测人体汗液中的肌酐和皮质醇。所提出的SERS系统提供了拉曼光谱法，可以在小样本量（2 μ L）上进行，以识别正常和危险的肌酐和皮质醇组。
The conventional tissue biopsy method yields isolated snapshots of a narrow region. Therefore, it cannot facilitate comprehensive disease characterization and monitoring. Recently, the detection of tumor-derived components in body fluids-a practice known as liquid biopsy-has attracted increased attention from the biochemical research and clinical application viewpoints. In this vein, surface-enhanced Raman scattering (SERS) has been identified as one of the most powerful liquid-biopsy analysis techniques, owing to its high sensitivity and specificity. Moreover, it affords high-capacity spectral multiplexing for simultaneous target detection and a unique ability to obtain intrinsic biomolecule-fingerprint spectra. This paper presents the fabrication of silver nanosnowflakes (SNSFs) using the polyol method and their subsequent dropping onto a hydrophobic filter paper. The SERS substrate, which comprises the SNSFs and hydrophobic filter paper, facilitates the simultaneous detection of creatinine and cortisol in human sweat using a hand-held Raman spectrometer. The proposed SERS system affords Raman spectrometry to be performed on small sample volumes (2 mu L) to identify the normal and at-risk creatinine and cortisol groups.