Identification of diminished tissue culture invasiveness among multiple antibiotic resistant Salmonella typhimurium DT104
Identification of diminished tissue culture invasiveness among multiple antibiotic resistant Salmonella typhimurium DT104
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DOI：
10.1006/mpat.1999.0322
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发表时间：
2000-01-01
影响因子：
3.8
通讯作者：
Jones, BD
中科院分区：
文献类型：
--
作者：
Carlson, SA;Browning, M;Jones, BD
沙门氏菌感染继续在世界各地引起胃肠道和全身疾病。由于鼠伤寒沙门氏菌明显增强了获取多种抗生素耐药基因的能力，因此进一步构成了一个主要的健康问题。目前尚不清楚多重耐药鼠伤寒沙门氏菌比非耐药鼠伤寒沙门氏菌致病性更强还是更低。通过体外侵袭试验，我们评估了400多株多重耐药鼠伤寒沙门氏菌的相对致病性。我们的研究没有发现任何“超侵入性”的分离株。然而，我们确定了12株表现为侵袭性表型的菌株，这些菌株与对照相比受到限制。这些“低侵袭性”菌株在各种噬菌体类型中被发现，这些噬菌体类型都至少具有六抗特征。进一步的研究表明，侵袭性的改变并不是由于依从性的改变。对体内毒力的有限研究表明，对所检测的低侵袭性菌株来说，导致小鼠死亡的能力略有下降。这些结果表明，对于某些多重耐药鼠伤寒沙门氏菌来说，致病能力并没有增加，而是相当轻微地减弱。(C)2000年学术出版社。
Salmonella infections continue to cause gastrointestinal and systemic disease throughout the world. Salmonella typhimurium further poses a major health concern due to its apparent enhanced ability to acquire multiple antibiotic resistance genes. Currently it is unclear if multiresistant S. typhimurium are more or less pathogenic than non-resistant counterparts. Using an in vitro invasion assay, we evaluated the relative pathogenicity of over 400 multiresistant S. typhimurium isolates. Our studies failed to identify any "hyperinvasive" isolates. However, we identified 12 isolates exhibiting invasive phenotypes that were constrained relative to controls. These "hypoinvasive" strains were found in a variety of phagetypes all possessing at least a hexaresistant profile. Further studies revealed that the alterations in invasion were not due to changes in adherence. Limited studies exploring in vivo virulence revealed a mildly decreased ability to cause murine lethality for the hypoinvasive strain examined. These results indicate that the ability to cause disease is not increased but is rather mildly attenuated for certain isolates of multiresistant S. typhimurium. (C) 2000 Academic Press.