LEFT RIGHT PAIRING AT THE CREST SYNAPSES OF RAT INTERPEDUNCULAR NUCLEUS
LEFT RIGHT PAIRING AT THE CREST SYNAPSES OF RAT INTERPEDUNCULAR NUCLEUS
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DOI：
10.1016/0306-4522(83)90301-9
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发表时间：
1983-01-01
期刊：
影响因子：
3.3
通讯作者：
HAMILL, GS
中科院分区：
文献类型：
--
作者：
LENN, NJ;WONG, V;HAMILL, GS
用电镜放射自显影法观察了大鼠脚间核内缰核-脚间核轴突的突触终末。终末有S型和脊型两种类型。脚间核的脊突触由2个缰核传入纤维与狭窄的树突接触组成。两个缰核注射放射性标记的氨基酸导致在48%的个别嵴突触的两个轴突的放射自显影标记，和39%的1个轴突。当只有1个缰被注射，不超过1个轴突被标记在个别嵴突触，这发生在59%。使用该方法的检测率（标记的末端与总末端的百分比）可以解释未标记的末端。可以针对双侧注射情况确定的检测率允许重新计算单侧注射情况的原始数据。缰传入神经的左右配对实际上发生在. apprx。90%的嵴突触。以这种方式使用检测率，另外通过EM变性研究的分析举例说明，在这两种实验神经解剖学方法的许多其他应用中也很重要，特别是当进行2组数据之间的比较时。对没有考虑到这一问题的研究结论必须谨慎解释。
Synaptic terminals of the habenulo-interpeduncular axons in rat interpeduncular nucleus were demonstrated by EM autoradiography following [3H]leucine injection into the habenula. The endings are of 2 types, S and crest synapses. The crest synapses in the interpeduncular nucleus consist of 2 habenulae afferents contacting a narrowed dendrite. Injection of both habenulae with radiolabeled amino acid resulted in autoradiographic labeling of both axons at 48% of individual crest synapses, and of 1 axon at 39%. When only 1 habenula was injected, no more than 1 axon was labeled at individual crest synapses; this occurred at 59%. Interpretation of unlabeled endings is possible using the detection rate of the method (% ratio of labeled to total endings). The detection rate, which can be determined for the bilateral injection case, allows recalculation of the raw data for the unilateral injection case. Left-right pairing of habenular afferents actually occurs at .apprx. 90% of crest synapses. The use of detection rates in this way, additionallly exemplified by analysis of an EM degeneration study, is also important in many other applications of both of these experimental neuroanatomical methods, especially when comparisons between 2 sets of data are made. The conclusions of studies which have not taken this problem into account must be interpreted with caution.