Multicolor In Vivo Imaging of Upconversion Nanopaticles with Emissions Tuned by Luminescence Resonance Energy Transfer
Multicolor In Vivo Imaging of Upconversion Nanopaticles with Emissions Tuned by Luminescence Resonance Energy Transfer
复制标题
通过发光共振能量转移调节发射的上转换纳米颗粒的多色体内成像
DOI：
10.1021/jp111006z
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发表时间：
2011-02-17
影响因子：
3.7
通讯作者：
Liu, Zhuang
中科院分区：
文献类型：
--
作者：
Cheng, Liang;Yang, Kai;Liu, Zhuang
合成了基于氟化钇钠（NaYF 4）纳米晶体的上转换纳米颗粒（UCNPs），用两亲聚合物官能化，并通过物理吸附负载荧光和猝灭分子。在近红外（NIR）激发下，纳米粒子与有机染料之间发生了发光共振能量转移（LRET），形成的超分子UCNP-染料复合物显示出可调谐的可见发射光谱，经光谱解卷积后，在可见光成像中可以很好地分离。我们的工作提供了一个简单和灵活的方法来调节UCNPs的上转换发光（UCL）光谱在动物体内的UCL成像。
Upconversion nanoparticles (UCNPs) based on sodium yttrium fluoride (NaYF4) nanocrystals are synthesized, functionalized with an amphiphilic polymer, and loaded with fluorescent and quenching molecules by physical adsorption. The formed supramolecular UCNP-dye complexes show tuned visible emission spectra owing to the luminescence resonance energy transfer (LRET) from nanoparticles to the organic dyes under near-infrared (NIR) excitation, and can be well separated in multicolor imaging after spectral decovolution. Our work provides a facile and flexible method to modulate the upconversion luminescence (UCL) spectra of UCNPs for in vivo multicolor UCL imaging in animals.