Mixer-settler counter-current chromatography with multiple spiral disk assembly
Mixer-settler counter-current chromatography with multiple spiral disk assembly
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DOI：
10.1016/j.chroma.2007.09.078
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发表时间：
2007-11-23
影响因子：
4.1
通讯作者：
Powell, Jimmie
中科院分区：
文献类型：
--
作者：
Ito, Yoichiro;Clary, Robert;Powell, Jimmie
开发了一种新型的高速逆流色谱系统，用于生物聚合物的分离。螺旋盘组件由8个单元组成，每个单元配备300多个混合-沉淀装置，并在固定相保留和分离效率方面进行了性能评估。采用聚乙二醇1000 （12.5%,w/w）和磷酸二氢钾（12.5%,w/w）组成的聚合物相体系，在不同流速和转速的实验条件下，使用两种稳定的肌红蛋白和溶菌酶蛋白样品进行了一系列实验。当转速为800-1000 rpm，流速为0.25-0.5 mi/min时，获得了最佳结果，其中100个理论板的分配效率达到了50%以上的固定相保留。Elsevier B.V.出版
A novel system for performing high-speed counter-current chromatography has been developed for separation of biopolymers using polymer phase systems. The spiral disk assembly consisting of eight units, each equipped with over 300 mixer-settler sets, was constructed and performance evaluated in terms of retention of the stationary phase and separation efficiency. A series of experiments was performed with a polymer phase system composed of polyethylene glycol 1000 (12.5%, w/w) and dibasic potassium phosphate (12.5%, w/w) using two stable protein samples of myoglobin and lysozyme at various experimental conditions of flow rates and revolution speeds. The best results were obtained with revolution speeds of 800-1000 rpm at flow rates of 0.25-0.5 mi/min where the partition efficiency of several 100 theoretical plates was achieved with over 50% stationary phase retention. Published by Elsevier B.V.